Toxicokinetics of naringin and its metabolite naringenin after 180-day repeated oral administration in beagle dogs assayed by a rapid resolution liquid chromatography/tandem mass spectrometric method

• Published in: Journal of asian natural products research 2012, Vol.14 (1), p.68-75
• Main Authors: Yang, Cui-Ping, Liu, Meng-Hua, Zou, Wei, Guan, Xiao-Ling, Lai, Li, Su, Wei-Wei
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Beagle; beagle dogs; Biological and medical sciences; Chromatography, Liquid; Citrus - chemistry; Dogs; dose response; Drug toxicity and drugs side effects treatment; Female; females; Flavanones - blood; Flavanones - chemistry; Flavanones - pharmacokinetics; General pharmacology; ionization; liquid chromatography; Male; males; mass spectrometry; Medical sciences; metabolites; Miscellaneous (drug allergy, mutagens, teratogens...); naringenin; naringin; oral administration; Pharmacognosy. Homeopathy. Health food; pharmacokinetics; Pharmacology. Drug treatments; safety assessment; Spectrometry, Mass, Electrospray Ionization; toxicokinetics; toxicology
• ISSN: 1477-2213; 1028-6020; 1477-2213
• DOI: 10.1080/10286020.2011.632369

Toxicokinetic characteristics of naringin and its metabolite naringenin were investigated in beagle dogs after oral administration of naringin at the doses of 20, 100, or 500 mg/kg/day in a repeated-dose study for 1, 30, 90, and 180 days. Plasma concentrations of naringin and naringenin were determined by a rapid resolution liquid chromatography/electrospray ionization/tandem mass spectrometric method. The results showed that no differences in systemic exposure were observed between male and female beagle dogs. Systematic exposure exhibited dose-dependent increase for both naringin and naringenin. No significant accumulations were observed. Results would be taken into consideration for the interpretation of toxicology findings and provide a reference for clinical safety assessment.