Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII

Hypertriglyceridemia has recently been considered to be an independent risk factor for coronary heart disease, in which apolipoprotein (Apo)CIII is one of the major contributory factors, as it is strongly correlated with plasma triglyceride levels. Although ApoCIII transgenic mice have been generate...

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Published in:The FEBS journal 2012-01, Vol.279 (1), p.91-99
Main Authors: Wei, Jingyuan, Ouyang, Hongsheng, Wang, Yuhui, Pang, Daxin, Cong, Nathan X., Wang, Tiedong, Leng, Bingfeng, Li, Dong, Li, Xiaoping, Wu, Rong, Ding, Yu, Gao, Fei, Deng, Yanhong, Liu, Bo, Li, Ziyi, Lai, Liangxue, Feng, Haihua, Liu, George, Deng, Xuming
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Apolipoprotein C-III - physiology
apolipoprotein CIII
Blotting, Western
Female
Fluorescent Antibody Technique
Humans
hypertriglyceridemia
Hypertriglyceridemia - etiology
Hypertriglyceridemia - metabolism
Hypertriglyceridemia - pathology
Immunoenzyme Techniques
Intestines - cytology
Intestines - metabolism
Lipids - blood
Lipoproteins - blood
Liver - cytology
Liver - metabolism
Male
Mice
miniature pig
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Sus scrofa
transgenic
Triglycerides - metabolism
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Summary:Hypertriglyceridemia has recently been considered to be an independent risk factor for coronary heart disease, in which apolipoprotein (Apo)CIII is one of the major contributory factors, as it is strongly correlated with plasma triglyceride levels. Although ApoCIII transgenic mice have been generated as an animal model for the study of hypertriglyceridemia, the features of lipoprotein metabolism in mice differ greatly from those in humans. Because of the great similarity between pigs and humans with respect to lipid metabolism and cardiovascular physiology, we generated transgenic miniature pigs expressing human ApoCIII by the transfection of somatic cells combined with nuclear transfer. The expression of human ApoCIII was detected in the liver and intestine of the transgenic pigs. As compared with nontransgenic controls, transgenic pigs showed significantly increased plasma triglyceride levels (83 ± 36 versus 38 ± 4 mg·dL−1, P 
ISSN:1742-464X
1742-4658
DOI:10.1111/j.1742-4658.2011.08401.x