Selective Protein Separation Using Siliceous Materials with a Trimethoxysilane-Containing Glycopolymer

• Published in: ACS applied materials & interfaces 2012-01, Vol.4 (1), p.411-417
• Main Authors: Seto, Hirokazu, Ogata, Yutaro, Murakami, Tatsuya, Hoshino, Yu, Miura, Yoshiko
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Cattle; Humans; Mannose - chemistry; Polymers - chemical synthesis; Polymers - chemistry; Proteins - chemistry; Proteins - isolation & purification; Silanes - chemistry
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/am2014713

A copolymer with α-d-mannose (Man) and trimethoxysilane (TMS) units was synthesized for immobilization on siliceous matrices such as a sensor cell and membrane. Immobilization of the trimethoxysilane-containing copolymer on the matrices was readily performed by incubation at high heat. The recognition of lectin by poly(Man-r-TMS) was evaluated by measurement with a quartz crystal microbalance (QCM) and adsorption on an affinity membrane, QCM results showed that the mannose-binding protein, concanavalin A, was specifically bound on a poly(Man-r-TMS)-immobilized cell with a higher binding constant than bovine serum albumin. The amount of concanavalin A adsorbed during permeation through a poly(Man-r-TMS)-immobilized membrane was higher than that through an unmodified membrane. Moreover, the concanavalin A adsorbed onto the poly(Man-r-TMS)-immobilized membrane was recoverable by permeation of a mannose derivative at high concentration.