Synthesis, spectroscopic study and anticancer activity of a water-soluble Nb(V) peroxo complex

We synthesized, characterized and studied the anticancer properties of a new water-soluble peroxo niobium complex (K3[Nb(Asc)(O2)3]·4H2O (Asc = ascorbate anion C6H6O62−)), as well as that of ascorbic acid, in human leukemic cells. The complex was synthesized and characterized by elemental, IR, Raman...

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Bibliographic Details
Published in:Journal of inorganic biochemistry 2011-02, Vol.105 (2), p.155-163
Main Authors: Thomadaki, Hellinida, Lymberopoulou-Karaliota, Alexandra, Maniatakou, Adamantia, Scorilas, Andreas
Format: Article
Language:English
Subjects:
Antineoplastic Agents - chemical synthesis
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Ascorbic acid
Ascorbic Acid - pharmacology
Cancer
Cell Survival - drug effects
Chemotherapy
Coordination Complexes - chemical synthesis
Coordination Complexes - chemistry
Coordination Complexes - pharmacology
Crystallography, X-Ray
Cytotoxic drugs
Drug Design
Drug Discovery
HL-60 Cells
Humans
Inhibitory Concentration 50
K562 Cells
Leukemia
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Conformation
Niobium
Spectrophotometry, Infrared
Spectrum Analysis, Raman
Thermogravimetry
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Summary:We synthesized, characterized and studied the anticancer properties of a new water-soluble peroxo niobium complex (K3[Nb(Asc)(O2)3]·4H2O (Asc = ascorbate anion C6H6O62−)), as well as that of ascorbic acid, in human leukemic cells. The complex was synthesized and characterized by elemental, IR, Raman, thermogravimetric analysis, detailed NMR and mass spectra analysis. The cytotoxic activity of the complex on HL-60 and K562 human leukemia cell lines has been investigated by assessing vital cellular mechanisms, such as the metabolic activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MTT) and the proliferation capacity (growth curves) of leukemia cells, as well as the structural integrity of cell membrane (trypan blue assay). The complex exerts an increased antiproliferative effect primarily on HL60 human leukemia cells, compared to ascorbic acid alone, as well as an inhibitory effect on necrosis caused by ascorbic acid. Its effect on K562 cells concerns mainly its inhibitory effect upon cell necrosis induced by ascorbic acid alone. Our results support a concentration- and time-dependent enhanced antileukemic effect of the complex, suggesting its significance as a promising tool in the confrontation of leukemia. [Display omitted]
ISSN:0162-0134
1873-3344
DOI:10.1016/j.jinorgbio.2010.10.012