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Multiple buds are an alternative target to genetic manipulation of kenaf ( Hibiscus cannabinus L.)
► Multiple buds are target for genetic modification driven by Agrobacterium tumefaciens. ► Thidiazuron induces multiple meristematic cell centres from kenaf mature seeds. ► Fertile transgenic plants generating transgenic progenies were also obtained. Kenaf ( Hibiscus cannabinus L.) is an annual fibe...
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Published in: | Industrial crops and products 2011-07, Vol.34 (1), p.937-942 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | ► Multiple buds are target for genetic modification driven by
Agrobacterium tumefaciens. ► Thidiazuron induces multiple meristematic cell centres from kenaf mature seeds. ► Fertile transgenic plants generating transgenic progenies were also obtained.
Kenaf (
Hibiscus cannabinus L.) is an annual fiber crop cultivated as an important source of cellulose and lignin. In the recent years, fibers have increased their economic importance as organic component in bio-composite materials. However, industry requires rheological improvements of these fibers. Genetic manipulation has been considered a feasible approach for fiber improvement; however, kenaf is considered a crop species difficult to manipulate. Here we propose meristematic cells from mature embryos as target for genetic modification driven by
Agrobacterium tumefaciens.
In vitro meristems proliferation and multiple shoots regeneration were evaluated by sowing kenaf mature seeds on medium containing three different concentration of thidiazuron, a substituted urea compound. The highest number of regenerated shoots was obtained from mature seeds germinated in presence of 10
μM thidiazuron after 14 days of culture. Interactions between two
A. tumefaciens strains and two kenaf varieties were assessed. Transgene stable integration and its inheritance in T
1 generation were also verified, demonstrating that kenaf meristematic cells are an useful target for genetic manipulation by agroinfection. |
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ISSN: | 0926-6690 1872-633X |
DOI: | 10.1016/j.indcrop.2011.02.016 |