Protective immunity to DENV2 after immunization with a recombinant NS1 protein using a genetically detoxified heat-labile toxin as an adjuvant

• Published in: Vaccine 2012-01, Vol.30 (5), p.837-845
• Main Authors: Amorim, Jaime Henrique, Diniz, Mariana Oliveira, Cariri, Francisco A.M.O, Rodrigues, Juliana Falcão, Bizerra, Raíza Sales Pereira, Gonçalves, Antônio J.S, de Barcelos Alves, Ada Maria, de Souza Ferreira, Luís Carlos
• Format: Article
• Language: English
• Subjects: Adjuvants; Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - adverse effects; Adjuvants, Immunologic - genetics; adverse effects; Allergy and Immunology; alum; aluminum hydroxide; Aluminum Hydroxide - administration & dosage; Amino acids; Animals; antibodies; Antibodies, Viral - blood; antigens; Bacterial Toxins - administration & dosage; Bacterial Toxins - adverse effects; Bacterial Toxins - genetics; blood serum; C-reactive protein; coagulation; Dengue - mortality; Dengue - pathology; Dengue fever; Dengue Vaccines - administration & dosage; Dengue Vaccines - adverse effects; Dengue Vaccines - immunology; Dengue virus; Dengue Virus - genetics; Dengue Virus - immunology; Deoxyribonucleic acid; DNA; Encephalitis; enterotoxigenic Escherichia coli; Enterotoxins - administration & dosage; Enterotoxins - adverse effects; Enterotoxins - genetics; Escherichia coli; Escherichia coli Proteins - administration & dosage; Escherichia coli Proteins - adverse effects; Escherichia coli Proteins - genetics; Freund's Adjuvant - administration & dosage; Heat-labile toxin; Hematology; hemorrhage; Humans; Immune response; Immune system; Immunization; immunoglobulin G; Immunology; Infections; Injections, Subcutaneous; Laboratory animals; Lymphocytes; Mice; Mice, Inbred BALB C; NS1 protein; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Studies; Survival Analysis; T-lymphocytes; T-Lymphocytes - immunology; Toxins; Toxoids - administration & dosage; Toxoids - adverse effects; Toxoids - genetics; Tropical diseases; Vaccines; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - adverse effects; Vaccines, Synthetic - immunology; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - immunology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2011.12.034

Highlights ► A subunit vaccine with DENV2 NS1 as antigen was proposed. ► LTG33D was used as an adjuvant for the first time in a DENV vaccine formulation. ► Antibodies with high affinity to DENV2 NS1 were generated. ► The immune response conferred protection of 50% under lethal challenge. ► The proposed vaccine did not elicited side effects in immunized mice.