Material properties and electrical stimulation regimens of polycaprolactone fumarate–polypyrrole scaffolds as potential conductive nerve conduits

The mechanical and electrical properties of polycaprolactone fumarate–polypyrrole (PCLF–PPy) scaffolds were studied under physiological conditions to evaluate their ability to maintain the material properties necessary for application as conductive nerve conduits. PC12 cells cultured on PCLF–PPy sca...

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Bibliographic Details
Published in:Acta biomaterialia 2011-03, Vol.7 (3), p.944-953
Main Authors: Moroder, Philipp, Runge, M. Brett, Wang, Huan, Ruesink, Terry, Lu, Lichun, Spinner, Robert J., Windebank, Anthony J., Yaszemski, Michael J.
Format: Article
Language:English
Subjects:
Adsorption
Animals
Biocompatible Materials
Calorimetry, Differential Scanning
cultured cells
electric current
Electric Stimulation
electrical properties
Electrical stimulation
electrical treatment
fluorescence microscopy
in vitro studies
mechanical properties
Nerve
nerve tissue
Neural Conduction
neurites
PC12 Cells
Polycaprolactone fumarate
Polyesters - chemistry
Polymers - chemistry
Polypyrrole
Proteins - chemistry
Pyrroles - chemistry
Rats
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Summary:The mechanical and electrical properties of polycaprolactone fumarate–polypyrrole (PCLF–PPy) scaffolds were studied under physiological conditions to evaluate their ability to maintain the material properties necessary for application as conductive nerve conduits. PC12 cells cultured on PCLF–PPy scaffolds were stimulated with regimens of 10μA of either a constant or a 20Hz frequency current passed through the scaffolds for 1h per day. PC12 cellular morphologies were analyzed by fluorescence microscopy after 48h. PCLF–PPy scaffolds exhibited excellent mechanical properties at 37°C which would allow suturing and flexibility. The surface resistivity of the scaffolds was 2kΩ and the scaffolds were electrically stable during the application of electrical stimulation (ES). In vitro studies showed significant increases in the percentage of neurite bearing cells, number of neurites per cell and neurite length in the presence of ES compared with no ES. Additionally, extending neurites were observed to align in the direction of the applied current. This study shows that electrically conductive PCLF–PPy scaffolds possess the material properties necessary for application as nerve conduits. Additionally, the capability to significantly enhance and direct neurite extension by passing an electrical current through PCLF–PPy scaffolds renders them even more promising as future therapeutic treatments for severe nerve injuries.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2010.10.013