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PCR for rapid diagnosis of acute Q fever at a combat support hospital in Iraq
Acute Q fever is occasionally seen in U.S. military service members deployed to Iraq. Diagnosis relies on serology, which is not available in the combat zone. Improved diagnostic modalities are needed. We performed a pilot study investigating whether Joint Biological Agent Identification and Diagnos...
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| Published in: | Military medicine 2011-01, Vol.176 (1), p.103-105 |
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| Main Authors: | , , , , |
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| container_end_page | 105 |
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| container_title | Military medicine |
| container_volume | 176 |
| creator | Hamilton, Lanette R George, Dena L Scoville, Stephanie L Hospenthal, Duane R Griffith, Matthew E |
| description | Acute Q fever is occasionally seen in U.S. military service members deployed to Iraq. Diagnosis relies on serology, which is not available in the combat zone. Improved diagnostic modalities are needed. We performed a pilot study investigating whether Joint Biological Agent Identification and Diagnostic System (JBAIDS), a ruggedized, deployable polymerase chain reaction (PCR) platform, might be useful in the diagnosis of acute Q fever. Patients presenting to a Combat Support Hospital in Iraq with undifferentiated fever had blood drawn for Q fever PCR and these results were compared with serology. PCR was positive in 6 of 9 patients with acute Q fever by serology and negative in all 9 patients with negative serology. These results suggest that PCR using the JBAIDS platform could be of use in the diagnosis of Q fever in deployed settings. Further research into this modality is warranted. |
| doi_str_mv | 10.7205/MILMED-D-10-00111 |
| format | article |
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Diagnosis relies on serology, which is not available in the combat zone. Improved diagnostic modalities are needed. We performed a pilot study investigating whether Joint Biological Agent Identification and Diagnostic System (JBAIDS), a ruggedized, deployable polymerase chain reaction (PCR) platform, might be useful in the diagnosis of acute Q fever. Patients presenting to a Combat Support Hospital in Iraq with undifferentiated fever had blood drawn for Q fever PCR and these results were compared with serology. PCR was positive in 6 of 9 patients with acute Q fever by serology and negative in all 9 patients with negative serology. These results suggest that PCR using the JBAIDS platform could be of use in the diagnosis of Q fever in deployed settings. Further research into this modality is warranted.</description><identifier>ISSN: 0026-4075</identifier><identifier>EISSN: 1930-613X</identifier><identifier>DOI: 10.7205/MILMED-D-10-00111</identifier><identifier>PMID: 21305969</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adolescent ; Adult ; Armed forces ; Biological & chemical terrorism ; Diagnostic tests ; Effectiveness ; FDA approval ; Female ; Fever ; Hospitals ; Hospitals, Military ; Humans ; Iraq - epidemiology ; Iraq War, 2003-2011 ; Laboratories ; Male ; Military Personnel ; Military service ; Patients ; Pilot Projects ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Q Fever - diagnosis ; Q Fever - epidemiology ; Sample size ; Serology ; Thrombocytopenia ; United States</subject><ispartof>Military medicine, 2011-01, Vol.176 (1), p.103-105</ispartof><rights>Copyright Association of Military Surgeons of the United States Jan 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-c6b3cce0e992758f1b845732ea78bc120973764ebc20c1315907b7f57f902aae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21305969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamilton, Lanette R</creatorcontrib><creatorcontrib>George, Dena L</creatorcontrib><creatorcontrib>Scoville, Stephanie L</creatorcontrib><creatorcontrib>Hospenthal, Duane R</creatorcontrib><creatorcontrib>Griffith, Matthew E</creatorcontrib><title>PCR for rapid diagnosis of acute Q fever at a combat support hospital in Iraq</title><title>Military medicine</title><addtitle>Mil Med</addtitle><description>Acute Q fever is occasionally seen in U.S. military service members deployed to Iraq. 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Further research into this modality is warranted.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Armed forces</subject><subject>Biological & chemical terrorism</subject><subject>Diagnostic tests</subject><subject>Effectiveness</subject><subject>FDA approval</subject><subject>Female</subject><subject>Fever</subject><subject>Hospitals</subject><subject>Hospitals, Military</subject><subject>Humans</subject><subject>Iraq - epidemiology</subject><subject>Iraq War, 2003-2011</subject><subject>Laboratories</subject><subject>Male</subject><subject>Military Personnel</subject><subject>Military service</subject><subject>Patients</subject><subject>Pilot Projects</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Q Fever - diagnosis</subject><subject>Q Fever - epidemiology</subject><subject>Sample size</subject><subject>Serology</subject><subject>Thrombocytopenia</subject><subject>United States</subject><issn>0026-4075</issn><issn>1930-613X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkU9PwzAMxSMEYmPwAbigiAungp00TXNE24BJm_gjkLhVaZZCp63pkhaJb0_HBgcunGxZv_dk-xFyinApGYir2WQ6G4-iUYQQASDiHumj4hAlyF_3SR-AJVEMUvTIUQiLDolVioekx5CDUInqk9nD8IkWzlOv63JO56V-q1woA3UF1aZtLH2khf2wnuqGamrcKu-a0Na18w19d6EuG72kZUUnXq-PyUGhl8Ge7OqAvNyMn4d30fT-djK8nkYmBtZEJsm5MRasUkyKtMA8jYXkzGqZ5gYZKMllEtvcMDDIUSiQuSyELBQwrS0fkIutb-3durWhyVZlMHa51JV1bcgUpiAYw-RfMhUYy81XOvL8D7lwra-6M7I0TlncrSE6CLeQ8S4Eb4us9uVK-88MIdtkkm0zyUabwXcmneZsZ9zmKzv_VfyEwL8ABaeE8g</recordid><startdate>201101</startdate><enddate>201101</enddate><creator>Hamilton, Lanette R</creator><creator>George, Dena L</creator><creator>Scoville, Stephanie L</creator><creator>Hospenthal, Duane R</creator><creator>Griffith, Matthew E</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88F</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M1Q</scope><scope>M2M</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>201101</creationdate><title>PCR for rapid diagnosis of acute Q fever at a combat support hospital in Iraq</title><author>Hamilton, Lanette R ; George, Dena L ; Scoville, Stephanie L ; Hospenthal, Duane R ; Griffith, Matthew E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-c6b3cce0e992758f1b845732ea78bc120973764ebc20c1315907b7f57f902aae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Armed forces</topic><topic>Biological & chemical terrorism</topic><topic>Diagnostic tests</topic><topic>Effectiveness</topic><topic>FDA approval</topic><topic>Female</topic><topic>Fever</topic><topic>Hospitals</topic><topic>Hospitals, Military</topic><topic>Humans</topic><topic>Iraq - 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Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Military medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamilton, Lanette R</au><au>George, Dena L</au><au>Scoville, Stephanie L</au><au>Hospenthal, Duane R</au><au>Griffith, Matthew E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR for rapid diagnosis of acute Q fever at a combat support hospital in Iraq</atitle><jtitle>Military medicine</jtitle><addtitle>Mil Med</addtitle><date>2011-01</date><risdate>2011</risdate><volume>176</volume><issue>1</issue><spage>103</spage><epage>105</epage><pages>103-105</pages><issn>0026-4075</issn><eissn>1930-613X</eissn><abstract>Acute Q fever is occasionally seen in U.S. military service members deployed to Iraq. 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| ispartof | Military medicine, 2011-01, Vol.176 (1), p.103-105 |
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| source | Oxford Journals Online |
| subjects | Adolescent Adult Armed forces Biological & chemical terrorism Diagnostic tests Effectiveness FDA approval Female Fever Hospitals Hospitals, Military Humans Iraq - epidemiology Iraq War, 2003-2011 Laboratories Male Military Personnel Military service Patients Pilot Projects Polymerase chain reaction Polymerase Chain Reaction - methods Q Fever - diagnosis Q Fever - epidemiology Sample size Serology Thrombocytopenia United States |
| title | PCR for rapid diagnosis of acute Q fever at a combat support hospital in Iraq |
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