Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray

Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an...

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Bibliographic Details
Published in:Acta biomaterialia 2010-10, Vol.6 (10), p.3936-3946
Main Authors: Skottrup, Peter Durand, Hansen, Mikkel Fougt, Lange, Jacob Moresco, Deryabina, Maria, Svendsen, Winnie Edith, Jakobsen, Mogens Havsteen, Dufva, Martin
Format: Article
Language:English
Subjects:
Biosensing Techniques
Biotin
Biotin - chemistry
Biotin - metabolism
C-Reactive Protein - metabolism
Immunoassay - methods
Magnetic bead
Magnetics
Materials Testing
Microarray
Microarray Analysis - methods
Microspheres
Molecular Structure
Polyethylene Glycols - chemistry
Serum Albumin, Bovine - metabolism
Silica
Silicon Dioxide - chemistry
Streptavidin
Streptavidin - chemistry
Streptavidin - metabolism
Surface Properties
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Summary:Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO 2 with or without surface modification. SiO 2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized. Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry. APTS/glutaraldehyde, directly linked TsT and bare H 2O 2-activated SiO 2 performed better than polyethylene glycol-modified surfaces. Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/antigen interactions, illustrated by C-reactive protein, were best performed with Masterbeads. The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2010.04.019