Evaluation of the immunogenicity and in vivo toxicity of the antimicrobial peptide P34

• Published in: International journal of pharmaceutics 2011-12, Vol.421 (1), p.94-98
• Main Authors: de Almeida Vaucher, Rodrigo, de Campos Velho Gewehr, Camila, Folmer Correa, Ana Paula, Sant‘Anna, Voltaire, Ferreira, Juliano, Brandelli, Adriano
• Format: Article
• Language: English
• Subjects: acute toxicity; alanine transaminase; animal models; Animals; Antimicrobial peptide; antimicrobial peptides; Bacillus; Bacteriocin; Biological and medical sciences; blood chemistry; death; foods; Freund's Adjuvant - administration & dosage; General pharmacology; hypersensitivity; immune response; In vivo toxicity; Lethal Dose 50; liver; Liver - drug effects; Liver - pathology; Male; Medical sciences; megakaryocytes; Mice; Mice, Inbred BALB C; nisin; Nisin - toxicity; oral administration; Peptides - administration & dosage; Peptides - toxicity; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Skin - drug effects; Skin - pathology; spleen; Spleen - drug effects; Spleen - pathology; toxicity testing
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2011.09.020

Immunogenicity and toxicity of antimicrobial peptide P34 were evaluated in vivo. BALB/c mice were inoculated intraperitoneally with peptide P34 alone and associated with Freund's adjuvant. For acute toxicity testing, different concentrations of the peptide P34 (82.5, 165.0, 247.5 and 330.0mg/kg) were orally administered. To evaluate the sub-chronic toxicity the tested dose of 0.825mg/kg/day of the peptide P34 or nisin were administered for 21 days. There were no hypersensitivity reactions or significant increase in antibody titer during the immunogenicity experiment or death of animals during the acute or sub-chronic toxicity tests. The LD50 was higher than 332.3±0.76mg/kg. No significant changes in serum biochemical parameters were observed in the animals treated with the peptide P34 unlike nisin-treated group showed a significant increase in alanine transaminase levels in comparison to controls. The group treated with 0.825mg/kg/day of nisin showed histological changes in the spleen, skin and liver. In the group treated with peptide P34 histological changes in the spleen were observed, with the presence of megakaryocytes. Few studies report the use of animal models to evaluate the in vivo toxicity of antimicrobial peptides and such investigation is an essential step to ensure it safe use in foods.