Molecular identification of Bartonella quintana infection using species-specific real-time PCR targeting transcriptional regulatory protein ( bqtR) gene

We describe a SYBR Green I-based real-time PCR targeting Bartonella quintana transcriptional regulatory protein ( bqtR) gene, recently found as invariant gene among different B. quintana strains. Melting curve analysis allowed us to discriminate between B. quintana and Bartonella henselae amplified...

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Bibliographic Details
Published in:Molecular and cellular probes 2011-10, Vol.25 (5), p.238-242
Main Authors: Liberto, Maria Carla, Lamberti, Angelo G., Marascio, Nadia, Matera, Giovanni, Quirino, Angela, Barreca, Giorgio S., Baudi, Francesco, Focà, Alfredo
Format: Article
Language:English
Subjects:
Bacteremia
Bacterial Typing Techniques
Bartonella henselae
Bartonella henselae - genetics
Bartonella henselae - isolation & purification
Bartonella Infections - blood
Bartonella Infections - diagnosis
Bartonella Infections - microbiology
Bartonella quintana
Bartonella quintana - genetics
Bartonella quintana - isolation & purification
Base Sequence
bqtR
DNA, Bacterial - blood
Humans
Molecular Sequence Data
Real-time PCR
Real-Time Polymerase Chain Reaction - methods
Sensitivity and Specificity
Species Specificity
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Summary:We describe a SYBR Green I-based real-time PCR targeting Bartonella quintana transcriptional regulatory protein ( bqtR) gene, recently found as invariant gene among different B. quintana strains. Melting curve analysis allowed us to discriminate between B. quintana and Bartonella henselae amplified products. We also show its usefulness in the management of a blood culture-negative patient affected by enlarged cervical lymphonodes and long-lasting fever. B. quintana DNA detection in patient whole blood samples and blood culture bottles was confirmed by sequencing and analyzing amplified products.
ISSN:0890-8508
1096-1194
DOI:10.1016/j.mcp.2011.07.001