Microsatellite markers for Aspidosperma polyneuron (Apocynaceae), an endangered tropical tree species

• Premise of the study: This study was designed to develop and characterize a preliminary set of nuclear microsatellite markers for Aspidosperma polyneuron, an endangered tropical tree species. • Methods and Results: Leaf samples were collected from a group of mature A. polyneuron trees in a large a...

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Bibliographic Details
Published in:American journal of botany 2011-11, Vol.98 (11), p.e300-e302
Main Authors: Ferreira‐Ramos, Ronai, Monteiro, Mariza, Zucchi, Maria I., Pinheiro, José B., Martinez, Carlos A., Mestriner, Moacyr A., Alzate‐Marin, Ana Lilia
Format: Article
Language:English
Subjects:
Apocynaceae
Aspidosperma - classification
Aspidosperma - genetics
Aspidosperma polyneuron
Brazil
DNA, Plant - analysis
Endangered & extinct species
Endangered Species
forest conservation
Gene Flow
Gene loci
genetic diversity
Genetic markers
Genetic Variation
Microsatellite Repeats
peroba‐rosa
Polymorphism
Red List of Threatened Species
Rivers
Species Specificity
SSR
Trees
Trees - classification
Trees - genetics
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Summary:• Premise of the study: This study was designed to develop and characterize a preliminary set of nuclear microsatellite markers for Aspidosperma polyneuron, an endangered tropical tree species. • Methods and Results: Leaf samples were collected from a group of mature A. polyneuron trees in a large area along the Pardo and Mogi‐Guaçu river basins in São Paulo State in the southeastern region of Brazil. Twenty‐five simple sequence repeat (SSR) markers were developed and 16 of 30 samples had polymorphic loci. The number of alleles per locus ranged from two to 17 (average = 7.06). A high diversity (He = 0.65) and parentage exclusion probability for the first (0.9995) and second (0.9999) parents were measured. • Conclusions: These 16 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. polyneuron.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100222