Visible light-induced crosslinkable gelatin

A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, w...

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Bibliographic Details
Published in:Acta biomaterialia 2010-10, Vol.6 (10), p.4005-4010
Main Authors: Son, Tae Il, Sakuragi, Makoto, Takahashi, Sawa, Obuse, Sei, Kang, Jeonghwa, Fujishiro, Masako, Matsushita, Haruhiko, Gong, Jiansheng, Shimizu, Shigeru, Tajima, Yusuke, Yoshida, Yasuhiro, Suzuki, Kazuomi, Yamamoto, Toshio, Nakamura, Mariko, Ito, Yoshihiro
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
Biocompatible Materials - radiation effects
Biosealant
Cell Culture Techniques - methods
Cell Line
Cross-Linking Reagents - chemistry
Dental Pulp - cytology
Dental Pulp - metabolism
Direct pulp capping
Fluorescent Dyes - chemistry
Gelatin
Gelatin - chemistry
Gelatin - radiation effects
Light
Materials Testing
Mice
Molecular Structure
Photo-crosslinking
Photochemistry - methods
Pulp Capping and Pulpectomy Agents - chemistry
Pulp Capping and Pulpectomy Agents - radiation effects
Rats
Rose Bengal - chemistry
Swine
Visible light
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Summary:A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, which is a visible light sensitizer. Irradiation by visible light solidified the aqueous solution. In addition, when the solution was cast on a plate, dried and photo-irradiated in the presence of a photomask a micropattern was formed that matched the micropattern on the photomask. The gelatin-immobilized regions enhanced cell adhesion. It was also confirmed that the gelatin incorporating furfuryl and Rose Bengal have no significant toxicity. The photo-crosslinkable gelatin was employed as a direct pulp capping material in the dental field. Considering these results, this system could be useful as a new type of visible light-induced crosslinkable biosealant.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2010.05.018