In vivo innate immune responses of groper ( Polyprion oxygeneios) against Miamiensis avidus infection and lack of protection following dietary vitamin C administration

• Published in: Fish & shellfish immunology 2012, Vol.32 (1), p.8-15
• Main Authors: Salinas, Irene, Anderson, Sally A., Wright, Jonathan, Webb, Victoria L.
• Format: Article
• Language: English
• Subjects: Animals; Ascorbic Acid - immunology; Ciliophora Infections - immunology; Ciliophora Infections - pathology; Ciliophora Infections - prevention & control; Ciliophora Infections - veterinary; Diet - veterinary; Dietary Supplements; Fish Diseases - immunology; Fish Diseases - parasitology; Fish Diseases - pathology; Fish Diseases - prevention & control; Immunity, Innate; Miamiensis avidus; New Zealand groper ( Polyprion oxygeneios); Oligohymenophorea - immunology; Perciformes - immunology; Perciformes - parasitology; Peroxidase; Polyprion oxygeneios; Reactive oxygen species; Respiratory Burst - immunology; Vitamin C
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2011.08.017

Scuticociliates are extracellular histophagous parasites that affect farmed fish worldwide. One of the most common pathogenic species is Miamiensis avidus, a pathogen of New Zealand groper ( Polyprion oxygeneios). The aim of this study was to characterise both the host (groper)-parasite ( M. avidus) immune interactions and the possible protective role of dietary sodium ascorbate. Head-kidney leucocytes (HKLs) from naturally infected adult groper showed decreased respiratory burst response and peroxidase (Px) levels than healthy individuals. Infected groper also had significantly higher serum Px levels compared to controls. Myeloperoxidase (MPO) was inhibited in the head-kidney (HK) whereas MPO + cells were observed in the skin and muscle lesions. The inhibition of the innate immune responses was further studied in experimental infections with M. avidus, which confirmed depletion of Px inside leucocytes and marked increases in serum Px in infected individuals. Groper juveniles were fed a diet supplemented with sodium ascorbate (Vitamin C) (2g Kg −1) for 21 days and then challenged by subcutaneous injection or immersion exposure with live M. avidus cells. No protection was observed in the sodium ascorbate fed groper compared to the control diet following challenge by either injection or immersion. In vitro assays showed that sodium ascorbate itself results in the inhibition of Px and respiratory burst of groper HKLs, supporting the results obtained in vivo. Our results show that histophagous protozoa such as M. avidus hamper innate immune defences of fish hosts and that dietary sodium ascorbate does not protect groper against experimental infection with this parasite. ► Groper naturally infected with scuticociliates show decreased innate immune activity. ► Experimental infection with M. avidus inhibits leucocyte Px 13 days post infection. ► Dietary vitamin C did not protect groper against M. avidus challenge.