Inhibitory constituents of Nardostachys chinensis on nitric oxide production in RAW 264.7 macrophages

• Published in: Bioorganic & medicinal chemistry letters 2012-01, Vol.22 (1), p.706-708
• Main Authors: Hwang, Ji Sang, Lee, Seon A, Hong, Seong Su, Han, Xiang Hua, Lee, Chul, Lee, Dongho, Lee, Chong-Kil, Hong, Jin Tae, Kim, Youngsoo, Lee, Mi Kyeong, Hwang, Bang Yeon
• Format: Article
• Language: English
• Subjects: alcohols; Animals; Biological and medical sciences; Drug Design; fractionation; Inhibitory Concentration 50; Lipopolysaccharides - chemistry; macrophages; Macrophages - cytology; Magnetic Resonance Spectroscopy - methods; Medical sciences; Methanol - chemistry; Mice; Models, Chemical; Nardostachys - metabolism; Nardostachys chinensis; Nardostachys grandiflora; nitric oxide; Nitric Oxide - antagonists & inhibitors; Nitric Oxide - chemistry; Nitric Oxide - metabolism; Nitric oxide inhibitor; p-coumaric acid; Pharmacology. Drug treatments; Plant Extracts - pharmacology; Plant Roots - metabolism; Rhizome - chemistry; rhizomes; roots; Sesquiterpenoid; sesquiterpenoids; Spectrophotometry - methods; spectroscopy; ursolic acid; Valerianaceae
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2011.10.043

The activity-guided fractionation of the MeOH extract of the rhizomes and roots of Nardostachys chinensis led to the isolation of two new sesquiterpenoids, narchinol B (8) and narchinol C (9), along with 10 known compounds. Their structures were determined using spectroscopic techniques, which included 1D- and 2D-NMR. All of the isolates were evaluated for their potential to inhibit the LPS-induced NO production in murine macrophage RAW 264.7 cells. The activity-guided fractionation of the MeOH extract of the rhizomes and roots of Nardostachys chinensis led to the isolation of two new sesquiterpenoids, narchinol B (8) and narchinol C (9), along with 10 known compounds, ursolic acid (1), nardosinone (2), pinoresinol (3), desoxo-narchinol A (4), kanshone B (5), epoxyconiferyl alcohol (6), debilon (7), 4α,5-dimethyl-1,3-dioxo-1,2,3,4,4α,5,6,7-octahydronaphthalene (10), p-coumaric acid (11), and isoferulic acid (12). Their structures were determined using spectroscopic techniques, which included 1D- and 2D-NMR. Among the isolates, compounds 2, 4, 5, 8 and 9 showed inhibitory activity against LPS-induced NO production with IC50 values of 4.6–21.6μM.