Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis

Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGR...

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Published in:Fish & shellfish immunology 2012, Vol.32 (1), p.178-185
Main Authors: Wei, Xiumei, Yang, Jianmin, Yang, Dinglong, Xu, Jie, Liu, Xiangquan, Yang, Jialong, Fang, Jinghui, Qiao, Hongjin
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Bivalvia - genetics
Bivalvia - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cloning, Molecular
Gene Expression Profiling
Gene Expression Regulation
Hemocytes - metabolism
Innate immunity
Models, Molecular
Molecular Sequence Data
Mollusca
Pattern recognition receptor
PGRP
Phylogeny
Protein Structure, Tertiary
Real-time PCR
RNA, Messenger - genetics
Sequence Alignment
Solen grandis
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Summary:Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn 2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by β-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly up-regulated ( P 
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2011.11.009