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Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis
Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGR...
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| Published in: | Fish & shellfish immunology 2012, Vol.32 (1), p.178-185 |
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| container_title | Fish & shellfish immunology |
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| creator | Wei, Xiumei Yang, Jianmin Yang, Dinglong Xu, Jie Liu, Xiangquan Yang, Jialong Fang, Jinghui Qiao, Hongjin |
| description | Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk
Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn
2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by β-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly up-regulated (
P |
| doi_str_mv | 10.1016/j.fsi.2011.11.009 |
| format | article |
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Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn
2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by β-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly up-regulated (
P < 0.01) when
S.
grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of
S.
grandis, and they might perform different functions in the immune defense against invaders.
► We identified two short PGRPs from
Solen grandis. ► SgPGRP-S1 and SgPGRP-S2 exhibited different expression pattern in tissues. ► Both two PGRPs could be induced by stimulation of PGN, as well as other PAMPs. ► Results suggested SgPGRP-S1 and SgPGRP-S2 might serve as multi-specific PRRs.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2011.11.009</identifier><identifier>PMID: 22119574</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Bivalvia - genetics ; Bivalvia - metabolism ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cloning, Molecular ; Gene Expression Profiling ; Gene Expression Regulation ; Hemocytes - metabolism ; Innate immunity ; Models, Molecular ; Molecular Sequence Data ; Mollusca ; Pattern recognition receptor ; PGRP ; Phylogeny ; Protein Structure, Tertiary ; Real-time PCR ; RNA, Messenger - genetics ; Sequence Alignment ; Solen grandis</subject><ispartof>Fish & shellfish immunology, 2012, Vol.32 (1), p.178-185</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-7b05170d83865d1a3bdee93770ab2826744672cc5d842d7d751f876c71535d973</citedby><cites>FETCH-LOGICAL-c450t-7b05170d83865d1a3bdee93770ab2826744672cc5d842d7d751f876c71535d973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22119574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Xiumei</creatorcontrib><creatorcontrib>Yang, Jianmin</creatorcontrib><creatorcontrib>Yang, Dinglong</creatorcontrib><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Liu, Xiangquan</creatorcontrib><creatorcontrib>Yang, Jialong</creatorcontrib><creatorcontrib>Fang, Jinghui</creatorcontrib><creatorcontrib>Qiao, Hongjin</creatorcontrib><title>Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk
Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn
2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by β-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly up-regulated (
P < 0.01) when
S.
grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of
S.
grandis, and they might perform different functions in the immune defense against invaders.
► We identified two short PGRPs from
Solen grandis. ► SgPGRP-S1 and SgPGRP-S2 exhibited different expression pattern in tissues. ► Both two PGRPs could be induced by stimulation of PGN, as well as other PAMPs. ► Results suggested SgPGRP-S1 and SgPGRP-S2 might serve as multi-specific PRRs.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Bivalvia - genetics</subject><subject>Bivalvia - metabolism</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cloning, Molecular</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Hemocytes - metabolism</subject><subject>Innate immunity</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Mollusca</subject><subject>Pattern recognition receptor</subject><subject>PGRP</subject><subject>Phylogeny</subject><subject>Protein Structure, Tertiary</subject><subject>Real-time PCR</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Solen grandis</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkcFu1DAURS0EoqXwAWyQd8Aiw7MT27FYVRW0lQpUBdZWxn6JPCR2aieF_j0eTWGJkJ5kL867tu4h5CWDDQMm3-02ffYbDoxtygDoR-SYgRaV1o16vL8LqBrZtEfkWc47AJC1hKfkiHPGtFDNMbn7FEe069glascYfBhoFxydbj6fUvw1J8zZx0BjT5efkc44L97FYby3XaAJbRyCX_bAnOKCPtA31-c312_pgAEz7VOc6BTHcc0_6NfyUKBDKvE-PydP-m7M-OLhPCHfP374dnZRXX05vzw7vapsI2Cp1BYEU-DaupXCsa7eOkRdKwXdlrdcqqaRilsrXNtwp5wSrG-VtIqJWjit6hPy-pBb_ne7Yl7M5LPFcewCxjUbzVqQmsP_kLzUKZguJDuQNsWcE_ZmTn7q0r1hYPZezM4UL2bvxZQpXsrOq4f0dTuh-7vxR0QB3h8ALG3ceUwmW4_BovOl5sW46P8R_xv_h52a</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Wei, Xiumei</creator><creator>Yang, Jianmin</creator><creator>Yang, Dinglong</creator><creator>Xu, Jie</creator><creator>Liu, Xiangquan</creator><creator>Yang, Jialong</creator><creator>Fang, Jinghui</creator><creator>Qiao, Hongjin</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>2012</creationdate><title>Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis</title><author>Wei, Xiumei ; Yang, Jianmin ; Yang, Dinglong ; Xu, Jie ; Liu, Xiangquan ; Yang, Jialong ; Fang, Jinghui ; Qiao, Hongjin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-7b05170d83865d1a3bdee93770ab2826744672cc5d842d7d751f876c71535d973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Bivalvia - genetics</topic><topic>Bivalvia - metabolism</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cloning, Molecular</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Hemocytes - metabolism</topic><topic>Innate immunity</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Mollusca</topic><topic>Pattern recognition receptor</topic><topic>PGRP</topic><topic>Phylogeny</topic><topic>Protein Structure, Tertiary</topic><topic>Real-time PCR</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Solen grandis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Xiumei</creatorcontrib><creatorcontrib>Yang, Jianmin</creatorcontrib><creatorcontrib>Yang, Dinglong</creatorcontrib><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Liu, Xiangquan</creatorcontrib><creatorcontrib>Yang, Jialong</creatorcontrib><creatorcontrib>Fang, Jinghui</creatorcontrib><creatorcontrib>Qiao, Hongjin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Xiumei</au><au>Yang, Jianmin</au><au>Yang, Dinglong</au><au>Xu, Jie</au><au>Liu, Xiangquan</au><au>Yang, Jialong</au><au>Fang, Jinghui</au><au>Qiao, Hongjin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2012</date><risdate>2012</risdate><volume>32</volume><issue>1</issue><spage>178</spage><epage>185</epage><pages>178-185</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk
Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn
2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by β-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly up-regulated (
P < 0.01) when
S.
grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of
S.
grandis, and they might perform different functions in the immune defense against invaders.
► We identified two short PGRPs from
Solen grandis. ► SgPGRP-S1 and SgPGRP-S2 exhibited different expression pattern in tissues. ► Both two PGRPs could be induced by stimulation of PGN, as well as other PAMPs. ► Results suggested SgPGRP-S1 and SgPGRP-S2 might serve as multi-specific PRRs.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22119574</pmid><doi>10.1016/j.fsi.2011.11.009</doi><tpages>8</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection |
| subjects | Amino Acid Sequence Animals Base Sequence Bivalvia - genetics Bivalvia - metabolism Carrier Proteins - genetics Carrier Proteins - metabolism Cloning, Molecular Gene Expression Profiling Gene Expression Regulation Hemocytes - metabolism Innate immunity Models, Molecular Molecular Sequence Data Mollusca Pattern recognition receptor PGRP Phylogeny Protein Structure, Tertiary Real-time PCR RNA, Messenger - genetics Sequence Alignment Solen grandis |
| title | Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis |
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