In vitro and in vivo behaviour of zinc-doped phosphosilicate glasses

The aim of this work was to study the behaviour of zinc-doped phosphosilicate glasses based on Bioglass ® 45S5. In vitro (in simulated body fluid), the reactivity was analysed by means of inductively coupled plasma spectrometry, environmental scanning electron microscopy–energy-dispersive spectrosco...

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Bibliographic Details
Published in:Acta biomaterialia 2009, Vol.5 (1), p.419-428
Main Authors: Lusvardi, Gigliola, Zaffe, Davide, Menabue, Ledi, Bertoldi, Carlo, Malavasi, Gianluca, Consolo, Ugo
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Bioactive glasses
Calcium Phosphates - chemistry
Crystallization
Durapatite - chemistry
Environmental scanning electron microscopy (ESEM)
Glass - chemistry
In Vitro Techniques
In vitro test
In vivo test
Male
Mice
Microscopy, Electron, Scanning
Phosphorus Compounds - chemistry
Rats
Rats, Sprague-Dawley
Silicates - chemistry
Silicon Dioxide - chemistry
Time Factors
Zinc
Zinc - chemistry
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Summary:The aim of this work was to study the behaviour of zinc-doped phosphosilicate glasses based on Bioglass ® 45S5. In vitro (in simulated body fluid), the reactivity was analysed by means of inductively coupled plasma spectrometry, environmental scanning electron microscopy–energy-dispersive spectroscopy (ESEM–EDS) and X-ray diffraction. In vivo (a rat implanted with glass), the reactivity and the tissue behaviour were analysed by conventional histology, histochemistry, microradiography and ESEM–EDS. The in vivo behaviour matches that in vitro perfectly; they show comparable glass degradation processes and rates, ruled by the amount of zinc in the glass. The reaction mechanism for the formation of a polymerized silica layer superimposed with a peripheral calcium phosphate layer is clearly substantiated by ESEM–EDS investigations. The crystallization of a biologically active hydroxyapatite (HA) layer is observed in both cases; the in vitro experiment shows the presence of HA after 4 days.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2008.07.007