Obtention of plant peroxidase and its potential for the decolorization of the reactive dye Remazol Turquoise G 133

Peroxidases can be used in the decolorization process. There is a growing interest for new sources of this enzyme and for obtaining economically viable processes. In this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its opti...

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Bibliographic Details
Published in:Water science and technology 2012-01, Vol.65 (4), p.669-675
Main Authors: Silva, Maria Cristina, Torres, Juliana Arriel, Corrêa, Angelita Duarte, Junqueira, Allana Maria Bernardes, Amorim, Maria Teresa Pessoa, dos Santos, Custódio Donizete
Format: Article
Language:English
Subjects:
Acetone
Coloring Agents - chemistry
Coloring Agents - metabolism
Coordination Complexes - chemistry
Coordination Complexes - metabolism
Decoloring
Decolorization
Dyes
Enzyme Stability
Enzymes
Horseradish peroxidase
Hydrogen-Ion Concentration
Indoles - chemistry
Indoles - metabolism
Industrial Waste
Peroxidase
Peroxidases - chemistry
Peroxidases - metabolism
pH effects
Plant Proteins - chemistry
Plant Proteins - metabolism
Removal
Stability
Temperature
Textile Industry
Textile industry wastewaters
Waste Disposal, Fluid - methods
Water Pollutants, Chemical - chemistry
Water Pollutants, Chemical - metabolism
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Summary:Peroxidases can be used in the decolorization process. There is a growing interest for new sources of this enzyme and for obtaining economically viable processes. In this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its optimum pH, temperature, and stability conditions, and it is then applied in the decolorization of reactive dye Remazol Turquoise G 133%. The turnip peroxidase (TP) was utilized as an enzymatic source. This enzyme exhibited maximum activity at pH 7.0, and it was active in the temperature range of 30 to 50 °C, which favors its use in industrial processes. Acetone was the most efficient solvent to induce precipitation. The removal of Remazol Turquoise G 133% was 56.0% complete after 50 min, while 41.0% of the same dye was removed with the commercial horseradish peroxidase enzyme in 50 min. TP presents potential as a viable alternative in the decolorization of textile wastewaters.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2012.892