Short communication: Pheromonicin-SA affects mRNA expression of toll-like receptors, cytokines, and lactoferrin by Staphylococcus aureus-infected bovine mammary epithelial cells

• Published in: Journal of dairy science 2012-02, Vol.95 (2), p.759-764
• Main Authors: Zhu, Y.H., Liu, P.Q., Weng, X.G., Zhuge, Z.Y., Zhang, R., Ma, J.L., Qiu, X.Q., Li, R.Q., Zhang, X.L., Wang, J.F.
• Format: Article
• Language: English
• Subjects: Animal productions; Animals; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; antibacterial properties; Biological and medical sciences; bovine mastitis; Cattle; chemoattractants; cows; cytokine; Cytokines - analysis; Cytokines - biosynthesis; epithelial cells; Epithelium - microbiology; Female; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; gene expression; genes; Holstein; immune response; interleukin-1beta; Interleukin-1beta - analysis; Interleukin-1beta - biosynthesis; interleukin-6; Interleukin-6 - analysis; Interleukin-6 - biosynthesis; interleukin-8; Interleukin-8 - analysis; Interleukin-8 - biosynthesis; lactation; lactoferrin; Lactoferrin - analysis; Lactoferrin - biosynthesis; mammary glands; Mammary Glands, Animal - chemistry; Mammary Glands, Animal - microbiology; Mastitis, Bovine - drug therapy; Mastitis, Bovine - microbiology; messenger RNA; pheromonicin-SA; quantitative polymerase chain reaction; receptors; Recombinant Fusion Proteins - pharmacology; Recombinant Fusion Proteins - therapeutic use; rifampicin; RNA, Messenger - metabolism; Staphylococcal Infections - drug therapy; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcus aureus; Staphylococcus aureus - drug effects; Terrestrial animal productions; toll-like receptor; Toll-Like Receptor 2 - analysis; Toll-Like Receptor 2 - biosynthesis; Toll-Like Receptor 4 - analysis; Toll-Like Receptor 4 - biosynthesis; Toll-Like Receptors - analysis; Toll-Like Receptors - biosynthesis; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - biosynthesis; Vertebrates
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2011-4703

Pheromonicin-SA (Ph-SA) is a newly developed, engineered multidomain peptide that has a bactericidal effect against Staphylococcus aureus. The objective of this study was to characterize innate immune responses by Staph. aureus-stimulated bovine mammary epithelial cells (BMEC) following treatment with Ph-SA. Primary BMEC from one lactating Holstein cow were isolated and exposed to Staph. aureus for 2h, and then treated with rifampicin or Ph-SA. Total RNA was isolated from BMEC at 0, 2, 6, 12, and 24h postinfection, and the mRNA expression of selected genes, including toll-like receptor (TLR)2 and TLR4, IL-1β, IL-6, IL-8, tumor necrosis factor α (TNF-α), and lactoferrin, was quantified by real-time PCR. In the rifampicin group, increases in the expression of mRNA for TNF-α, IL-1β, IL-6, IL-8, and lactoferrin were observed at 6h postinfection and in the expression of mRNA for TLR2 but not for TLR4 at 12h postinfection. In the Ph-SA group, increases in the mRNA expression of TLR2, TNF-α, IL-1β, IL-6, IL-8, and lactoferrin were observed at 6h postinfection, and an increase in TLR4 mRNA expression was observed at 24h postinfection. At 24h postinfection, the mRNA expression of TLR4, TNF-α, IL-1β, IL-6, IL-8, and lactoferrin was higher in the Ph-SA group than in the rifampicin group. In conclusion, Ph-SA might promote the expression of mRNA for TLR2, TLR4, the pro-inflammatory cytokines IL-1, IL-6, and TNF-α, the chemotactic factor IL-8, and lactoferrin in Staph. aureus-infected BMEC. Moreover, Ph-SA may be of value as an antibiotic in promoting innate immune responses by Staph. aureus-infected bovine mammary epithelial cells.