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Multiplex cytokine analysis of aqueous humor in eyes with primary open-angle glaucoma, exfoliation glaucoma, and cataract
To measure levels of various inflammatory cytokines in the aqueous humor of patients with primary open-angle glaucoma (POAG), exfoliation glaucoma (EXG), and senile cataract. Aqueous humor samples were obtained from 64 eyes of 64 Japanese subjects (POAG, 20 eyes; EXG, 23 eyes; and cataract, 21 contr...
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Published in: | Investigative ophthalmology & visual science 2012-01, Vol.53 (1), p.241-247 |
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description | To measure levels of various inflammatory cytokines in the aqueous humor of patients with primary open-angle glaucoma (POAG), exfoliation glaucoma (EXG), and senile cataract.
Aqueous humor samples were obtained from 64 eyes of 64 Japanese subjects (POAG, 20 eyes; EXG, 23 eyes; and cataract, 21 control eyes). The levels of eight cytokines including interleukin (IL)1-β, IL-6, IL-8, transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α, serum amyloid A (SAA), migration inhibitory factor (MIF), and vascular endothelial growth factor (VEGF)-A were estimated using the multiplex bead immunoassay technique.
Compared with the cataract group, the levels of TGF-β1, IL-8, and SAA were significantly higher in aqueous humor samples from the POAG (5.0-fold, 2.3-fold, and 11.9-fold, respectively) and EXG (12.5-fold, 4.0-fold, and 18.3-fold, respectively) groups. Except for a significant decrease in the IL-6 level in the POAG (0.23-fold) group, no other cytokine levels differed in the POAG and EXG groups compared with the cataract group. The levels of TGF-β1, IL-8, and SAA were positively correlated with each other (ρ = 0.723-0.786; P < 0.0001), the intraocular pressure (IOP) (ρ = 0.392-0.662; P < 0.0001-0.0019), and the number of glaucoma medications (ρ = 0.478-0.659; P < 0.0001-0.0001).
Cytokine networks including TGF-β1, IL-8, and SAA in aqueous humor may have critical roles in IOP elevations in patients with open-angle glaucoma. |
doi_str_mv | 10.1167/iovs.11-8434 |
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Aqueous humor samples were obtained from 64 eyes of 64 Japanese subjects (POAG, 20 eyes; EXG, 23 eyes; and cataract, 21 control eyes). The levels of eight cytokines including interleukin (IL)1-β, IL-6, IL-8, transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α, serum amyloid A (SAA), migration inhibitory factor (MIF), and vascular endothelial growth factor (VEGF)-A were estimated using the multiplex bead immunoassay technique.
Compared with the cataract group, the levels of TGF-β1, IL-8, and SAA were significantly higher in aqueous humor samples from the POAG (5.0-fold, 2.3-fold, and 11.9-fold, respectively) and EXG (12.5-fold, 4.0-fold, and 18.3-fold, respectively) groups. Except for a significant decrease in the IL-6 level in the POAG (0.23-fold) group, no other cytokine levels differed in the POAG and EXG groups compared with the cataract group. The levels of TGF-β1, IL-8, and SAA were positively correlated with each other (ρ = 0.723-0.786; P < 0.0001), the intraocular pressure (IOP) (ρ = 0.392-0.662; P < 0.0001-0.0019), and the number of glaucoma medications (ρ = 0.478-0.659; P < 0.0001-0.0001).
Cytokine networks including TGF-β1, IL-8, and SAA in aqueous humor may have critical roles in IOP elevations in patients with open-angle glaucoma.</description><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.11-8434</identifier><identifier>PMID: 22159018</identifier><language>eng</language><publisher>United States</publisher><subject>Aged ; Aged, 80 and over ; Antihypertensive Agents - therapeutic use ; Aqueous Humor - metabolism ; Cataract - metabolism ; Cytokines - metabolism ; Exfoliation Syndrome - metabolism ; Female ; Glaucoma, Open-Angle - drug therapy ; Glaucoma, Open-Angle - metabolism ; Humans ; Immunoassay ; Intraocular Pressure ; Male ; Middle Aged ; Serum Amyloid A Protein - metabolism</subject><ispartof>Investigative ophthalmology & visual science, 2012-01, Vol.53 (1), p.241-247</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c290t-4f58ae062105a85f061356957c559e1f8915a776fb14e40a9d7a35fb1b53c8733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22159018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takai, Yasuyuki</creatorcontrib><creatorcontrib>Tanito, Masaki</creatorcontrib><creatorcontrib>Ohira, Akihiro</creatorcontrib><title>Multiplex cytokine analysis of aqueous humor in eyes with primary open-angle glaucoma, exfoliation glaucoma, and cataract</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To measure levels of various inflammatory cytokines in the aqueous humor of patients with primary open-angle glaucoma (POAG), exfoliation glaucoma (EXG), and senile cataract.
Aqueous humor samples were obtained from 64 eyes of 64 Japanese subjects (POAG, 20 eyes; EXG, 23 eyes; and cataract, 21 control eyes). The levels of eight cytokines including interleukin (IL)1-β, IL-6, IL-8, transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α, serum amyloid A (SAA), migration inhibitory factor (MIF), and vascular endothelial growth factor (VEGF)-A were estimated using the multiplex bead immunoassay technique.
Compared with the cataract group, the levels of TGF-β1, IL-8, and SAA were significantly higher in aqueous humor samples from the POAG (5.0-fold, 2.3-fold, and 11.9-fold, respectively) and EXG (12.5-fold, 4.0-fold, and 18.3-fold, respectively) groups. Except for a significant decrease in the IL-6 level in the POAG (0.23-fold) group, no other cytokine levels differed in the POAG and EXG groups compared with the cataract group. The levels of TGF-β1, IL-8, and SAA were positively correlated with each other (ρ = 0.723-0.786; P < 0.0001), the intraocular pressure (IOP) (ρ = 0.392-0.662; P < 0.0001-0.0019), and the number of glaucoma medications (ρ = 0.478-0.659; P < 0.0001-0.0001).
Cytokine networks including TGF-β1, IL-8, and SAA in aqueous humor may have critical roles in IOP elevations in patients with open-angle glaucoma.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antihypertensive Agents - therapeutic use</subject><subject>Aqueous Humor - metabolism</subject><subject>Cataract - metabolism</subject><subject>Cytokines - metabolism</subject><subject>Exfoliation Syndrome - metabolism</subject><subject>Female</subject><subject>Glaucoma, Open-Angle - drug therapy</subject><subject>Glaucoma, Open-Angle - metabolism</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Intraocular Pressure</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Serum Amyloid A Protein - metabolism</subject><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpNkD1PwzAQhi0EoqWwMSNvLA34kji2R1TxJRWxwBxdXac1OHGJE2j-PYlaUKd77_TopPch5BLYDUAmbq3_Dn2KZJqkR2QMnMcRFzI5PsgjchbCB2MxQMxOySiOgSsGcky6l9Y1duPMluqu8Z-2MhQrdF2wgfqC4ldrfBvoui19TW1FTWcC_bHNmm5qW2LdUb8xVYTVyhm6cthqX-KUmm3hncXG-urgitWSamywRt2ck5MCXTAX-zkh7w_3b7OnaP76-Dy7m0c6VqyJ0oJLNCyLgXGUvGAZJDxTXGjOlYFCKuAoRFYsIDUpQ7UUmPB-W_BES5EkE3K9-7upfV8mNHlpgzbOYTU0yxVILhQH1ZPTHalrH0JtinxfMQeWD67zwXWf8sF1j1_tH7eL0iz_4T-5yS89Tnw2</recordid><startdate>20120125</startdate><enddate>20120125</enddate><creator>Takai, Yasuyuki</creator><creator>Tanito, Masaki</creator><creator>Ohira, Akihiro</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120125</creationdate><title>Multiplex cytokine analysis of aqueous humor in eyes with primary open-angle glaucoma, exfoliation glaucoma, and cataract</title><author>Takai, Yasuyuki ; Tanito, Masaki ; Ohira, Akihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-4f58ae062105a85f061356957c559e1f8915a776fb14e40a9d7a35fb1b53c8733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antihypertensive Agents - therapeutic use</topic><topic>Aqueous Humor - metabolism</topic><topic>Cataract - metabolism</topic><topic>Cytokines - metabolism</topic><topic>Exfoliation Syndrome - metabolism</topic><topic>Female</topic><topic>Glaucoma, Open-Angle - drug therapy</topic><topic>Glaucoma, Open-Angle - metabolism</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Intraocular Pressure</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Serum Amyloid A Protein - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takai, Yasuyuki</creatorcontrib><creatorcontrib>Tanito, Masaki</creatorcontrib><creatorcontrib>Ohira, Akihiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takai, Yasuyuki</au><au>Tanito, Masaki</au><au>Ohira, Akihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiplex cytokine analysis of aqueous humor in eyes with primary open-angle glaucoma, exfoliation glaucoma, and cataract</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2012-01-25</date><risdate>2012</risdate><volume>53</volume><issue>1</issue><spage>241</spage><epage>247</epage><pages>241-247</pages><issn>1552-5783</issn><eissn>1552-5783</eissn><abstract>To measure levels of various inflammatory cytokines in the aqueous humor of patients with primary open-angle glaucoma (POAG), exfoliation glaucoma (EXG), and senile cataract.
Aqueous humor samples were obtained from 64 eyes of 64 Japanese subjects (POAG, 20 eyes; EXG, 23 eyes; and cataract, 21 control eyes). The levels of eight cytokines including interleukin (IL)1-β, IL-6, IL-8, transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α, serum amyloid A (SAA), migration inhibitory factor (MIF), and vascular endothelial growth factor (VEGF)-A were estimated using the multiplex bead immunoassay technique.
Compared with the cataract group, the levels of TGF-β1, IL-8, and SAA were significantly higher in aqueous humor samples from the POAG (5.0-fold, 2.3-fold, and 11.9-fold, respectively) and EXG (12.5-fold, 4.0-fold, and 18.3-fold, respectively) groups. Except for a significant decrease in the IL-6 level in the POAG (0.23-fold) group, no other cytokine levels differed in the POAG and EXG groups compared with the cataract group. The levels of TGF-β1, IL-8, and SAA were positively correlated with each other (ρ = 0.723-0.786; P < 0.0001), the intraocular pressure (IOP) (ρ = 0.392-0.662; P < 0.0001-0.0019), and the number of glaucoma medications (ρ = 0.478-0.659; P < 0.0001-0.0001).
Cytokine networks including TGF-β1, IL-8, and SAA in aqueous humor may have critical roles in IOP elevations in patients with open-angle glaucoma.</abstract><cop>United States</cop><pmid>22159018</pmid><doi>10.1167/iovs.11-8434</doi><tpages>7</tpages></addata></record> |
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subjects | Aged Aged, 80 and over Antihypertensive Agents - therapeutic use Aqueous Humor - metabolism Cataract - metabolism Cytokines - metabolism Exfoliation Syndrome - metabolism Female Glaucoma, Open-Angle - drug therapy Glaucoma, Open-Angle - metabolism Humans Immunoassay Intraocular Pressure Male Middle Aged Serum Amyloid A Protein - metabolism |
title | Multiplex cytokine analysis of aqueous humor in eyes with primary open-angle glaucoma, exfoliation glaucoma, and cataract |
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