Distribution of inflammatory cells, adhesion molecules, intermediate filaments, and chemokine receptors in subgroups of nasal polyp patients

• Published in: American journal of rhinology & allergy 2011-09, Vol.25 (5), p.e176-e180
• Main Authors: Onerci, Metin, Elsurer, Cagdas, Guzel, Elif E, Dagdeviren, Attila
• Format: Article
• Language: English
• Subjects: Adult; Antigens, CD - genetics; Antigens, CD - metabolism; Biomarkers - metabolism; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - immunology; Cell Adhesion Molecules - metabolism; Female; Gene Expression Profiling; Humans; Immunohistochemistry; Intermediate Filaments - genetics; Intermediate Filaments - immunology; Intermediate Filaments - metabolism; Male; Middle Aged; Nasal Polyps - immunology; Receptors, Chemokine - genetics; Receptors, Chemokine - immunology; Receptors, Chemokine - metabolism
• ISSN: 1945-8924; 1945-8932
• DOI: 10.2500/ajra.2011.25.3635

The pathogenesis of nasal polyps (NPs) is incompletely understood. The aim of this study was to investigate the distribution of inflammatory cells, adhesion molecules, intermediate filaments, and chemokine receptors in subgroups of NP patients. In total, 35 patients were enrolled (group 1, 10 patients with Samter syndrome; group 2, 10 patients with diffuse polyposis without signs of Samter syndrome; group 3: 5 patients with solitary nasal polyps; group 4, 10 controls). Immunohistochemical staining was performed for CD105, CD106, CD62E, CD4, CD8, CXCR4, CD147, CD90, CD104, BF45, vimentin, pancytokeratin, and muscle-specific actin (MSA) in all patients' specimens. Expression of CD4, CD8, and CD106 were similar between the groups. Number of patients expressing CD4 in groups 1, 2, and 3 were higher than the controls. Number of patients expressing CD8 antigen were significantly higher in all three groups than in the control group. Expression of CD147 in groups 3 and 4 was significantly higher than in groups 1 and 2. CD98 expression was higher in groups 1, 2, and 3 than in group 4. The number of patients expressing vimentin in groups 1, 2, and 3 was significantly higher than in group 4. Immunostaining for pancytokeratin was positive in all patients. In conclusion, inflammatory cell, adhesion molecule, intermediate filament, and chemokine receptor profiles in nasal polyps differ among different patient groups and control subjects. Additional specific immunohistochemical studies are necessary for development of more specific immunotherapies.