Super-resolution STED microscopy advances with yellow CW OPSL

Because these common fluorophores are optimized for blue excitation around 488 nm, this requires an optimum STED laser wavelength around 560-600 nm. For ultrafast pulses, this must be supplied by a optical parametric oscillator (QPO) pumped by a Ti:sapphire system. [...] an important development in...

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Bibliographic Details
Published in:Laser focus world 2012-01, Vol.48 (1), p.75
Main Authors: Honigmann, Alf, Eggeling, Christian, Schulze, Matthias, Lepert, Arnaud
Format: Magazinearticle
Language:English
Subjects:
Experiments
Lasers
Lipids
Microscopy
Noise
R&D
Research & development
Studies
Online Access:Get full text
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Summary:Because these common fluorophores are optimized for blue excitation around 488 nm, this requires an optimum STED laser wavelength around 560-600 nm. For ultrafast pulses, this must be supplied by a optical parametric oscillator (QPO) pumped by a Ti:sapphire system. [...] an important development in STED was to switch to the use of CW lasers.
ISSN:1043-8092