Loading…
Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples
The purpose of this study was to examine the performance of novel agars for the identification and enumeration of Campylobacter species. The analytical sensitivity and specificity of Campylobacter Selective agar (CASA), Brilliance CampyCount agar (BCCA) and CampyFoodIDagar (CFA) for 84 Campylobacter...
Saved in:
| Published in: | Journal of microbiological methods 2012-02, Vol.88 (2), p.304-310 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c388t-d76e8c58fe515d8d760cff5ddd193f2482a5d11678b8d91185fb85cbea91a0083 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c388t-d76e8c58fe515d8d760cff5ddd193f2482a5d11678b8d91185fb85cbea91a0083 |
| container_end_page | 310 |
| container_issue | 2 |
| container_start_page | 304 |
| container_title | Journal of microbiological methods |
| container_volume | 88 |
| creator | Ahmed, R. León-Velarde, C.G. Odumeru, J.A. |
| description | The purpose of this study was to examine the performance of novel agars for the identification and enumeration of Campylobacter species. The analytical sensitivity and specificity of Campylobacter Selective agar (CASA), Brilliance CampyCount agar (BCCA) and CampyFoodIDagar (CFA) for 84 Campylobacter spp. isolates and 50 non-Campylobacter spp. isolates from 37 distinct genera were of 100% sensitivity, with a 98% specificity for BCCA and CFA, and a 100% specificity for CASA. The application of these selective agars for Campylobacter spp. enumeration in comparison to the conventional agars, modified charcoal cefoperazonedeoxycholate agar (mCCDA) and Campy-Cefex (CCA) was examined using Campylobacter jejuni and Campylobacter coli inoculated samples. From C. jejuni inoculated samples, recovery on BCCA was significantly greater than other media (p0.05). With C. coli inoculated samples, recovery was significantly greater on BCCA and CASA than with other media (p |
| doi_str_mv | 10.1016/j.mimet.2011.12.011 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_919225917</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0167701211004374</els_id><sourcerecordid>919225917</sourcerecordid><originalsourceid>FETCH-LOGICAL-c388t-d76e8c58fe515d8d760cff5ddd193f2482a5d11678b8d91185fb85cbea91a0083</originalsourceid><addsrcrecordid>eNp90EFrFDEUB_Agil2rn0CQXMTTjHmZZidz8CBLW4WCF3ssIZO8aJbMZEwyC_vtTd213szlT-D3HsmfkLfAWmCw_bhvJz9haTkDaIG3NZ6RDcieN7ITw3Oyqapvegb8grzKec8YiO5KviQXvJ5tL7oNebg-6LDq4uNMo6NzPGCg-odOmbqYaPmJFOd1wvREdnpajiGO2hRMNC9LS_1Ml7iGko40YdE-0FxRwPyavHA6ZHxzzktyf3P9ffeluft2-3X3-a4xnZSlsf0WpRHSoQBhZb0y45yw1sLQOX4luRYW6mfkKO0AIIUbpTAj6gE0Y7K7JB9Oe5cUf62Yi5p8NhiCnjGuWQ0wcC4G6KvsTtKkmHNCp5bkJ52OCph6rFXt1Z9a1WOtCriqUafenfev44T2aeZvjxW8PwOdjQ4u6dn4_M8JwSVn2-o-nRzWNg4ek8rG42zQ-oSmKBv9fx_yGyp_l8I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>919225917</pqid></control><display><type>article</type><title>Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples</title><source>ScienceDirect Freedom Collection</source><creator>Ahmed, R. ; León-Velarde, C.G. ; Odumeru, J.A.</creator><creatorcontrib>Ahmed, R. ; León-Velarde, C.G. ; Odumeru, J.A.</creatorcontrib><description>The purpose of this study was to examine the performance of novel agars for the identification and enumeration of Campylobacter species. The analytical sensitivity and specificity of Campylobacter Selective agar (CASA), Brilliance CampyCount agar (BCCA) and CampyFoodIDagar (CFA) for 84 Campylobacter spp. isolates and 50 non-Campylobacter spp. isolates from 37 distinct genera were of 100% sensitivity, with a 98% specificity for BCCA and CFA, and a 100% specificity for CASA. The application of these selective agars for Campylobacter spp. enumeration in comparison to the conventional agars, modified charcoal cefoperazonedeoxycholate agar (mCCDA) and Campy-Cefex (CCA) was examined using Campylobacter jejuni and Campylobacter coli inoculated samples. From C. jejuni inoculated samples, recovery on BCCA was significantly greater than other media (p<0.05). Recovery on CASA was not significantly different from mCCDA and CCA (p>0.05). With C. coli inoculated samples, recovery was significantly greater on BCCA and CASA than with other media (p<0.05). The recovery of both C. jejuni and C. coli from inoculated samples with CFA was significantly less than with other media (P<0.05). CASA was able to effectively inhibit and differentiate Campylobacter spp. from background microflora while false positive organisms occurred with BCCA and CFA. An examination of 483 randomly selected suspect Campylobacter colonies from naturally contaminated samples demonstrated a colony confirmation rate for CCA, CFA, BCCA, mCCDA, and CASA, of 84%, 87%, 88%, 90%, and 100%, respectively. The media evaluated present an alternative to conventional selective agars for the identification and enumeration of thermotolerant Campylobacter spp. from samples of poultry origin through the farm to fork continuum.
► Assessed novel agars for the isolation and enumeration of Campylobacter spp. ► Agars were evaluated with the use of artificial and naturally contaminated samples. ► Two novel agars showed results comparable to conventional agars. ► False positive organisms found on both novel and conventional agars. ► CASA showed effective inhibition and differentiation of background flora.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2011.12.011</identifier><identifier>PMID: 22226753</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agar - chemistry ; Animals ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; BCCA ; Biological and medical sciences ; Campylobacter ; Campylobacter - isolation & purification ; CASA ; CCA ; CFA ; Chickens ; Colony Count, Microbial - methods ; Culture Media - chemistry ; Food Contamination - analysis ; Fundamental and applied biological sciences. Psychology ; mCCDA ; Meat - microbiology ; Microbiology ; Sensitivity and Specificity</subject><ispartof>Journal of microbiological methods, 2012-02, Vol.88 (2), p.304-310</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-d76e8c58fe515d8d760cff5ddd193f2482a5d11678b8d91185fb85cbea91a0083</citedby><cites>FETCH-LOGICAL-c388t-d76e8c58fe515d8d760cff5ddd193f2482a5d11678b8d91185fb85cbea91a0083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25528206$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22226753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahmed, R.</creatorcontrib><creatorcontrib>León-Velarde, C.G.</creatorcontrib><creatorcontrib>Odumeru, J.A.</creatorcontrib><title>Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>The purpose of this study was to examine the performance of novel agars for the identification and enumeration of Campylobacter species. The analytical sensitivity and specificity of Campylobacter Selective agar (CASA), Brilliance CampyCount agar (BCCA) and CampyFoodIDagar (CFA) for 84 Campylobacter spp. isolates and 50 non-Campylobacter spp. isolates from 37 distinct genera were of 100% sensitivity, with a 98% specificity for BCCA and CFA, and a 100% specificity for CASA. The application of these selective agars for Campylobacter spp. enumeration in comparison to the conventional agars, modified charcoal cefoperazonedeoxycholate agar (mCCDA) and Campy-Cefex (CCA) was examined using Campylobacter jejuni and Campylobacter coli inoculated samples. From C. jejuni inoculated samples, recovery on BCCA was significantly greater than other media (p<0.05). Recovery on CASA was not significantly different from mCCDA and CCA (p>0.05). With C. coli inoculated samples, recovery was significantly greater on BCCA and CASA than with other media (p<0.05). The recovery of both C. jejuni and C. coli from inoculated samples with CFA was significantly less than with other media (P<0.05). CASA was able to effectively inhibit and differentiate Campylobacter spp. from background microflora while false positive organisms occurred with BCCA and CFA. An examination of 483 randomly selected suspect Campylobacter colonies from naturally contaminated samples demonstrated a colony confirmation rate for CCA, CFA, BCCA, mCCDA, and CASA, of 84%, 87%, 88%, 90%, and 100%, respectively. The media evaluated present an alternative to conventional selective agars for the identification and enumeration of thermotolerant Campylobacter spp. from samples of poultry origin through the farm to fork continuum.
► Assessed novel agars for the isolation and enumeration of Campylobacter spp. ► Agars were evaluated with the use of artificial and naturally contaminated samples. ► Two novel agars showed results comparable to conventional agars. ► False positive organisms found on both novel and conventional agars. ► CASA showed effective inhibition and differentiation of background flora.</description><subject>Agar - chemistry</subject><subject>Animals</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>BCCA</subject><subject>Biological and medical sciences</subject><subject>Campylobacter</subject><subject>Campylobacter - isolation & purification</subject><subject>CASA</subject><subject>CCA</subject><subject>CFA</subject><subject>Chickens</subject><subject>Colony Count, Microbial - methods</subject><subject>Culture Media - chemistry</subject><subject>Food Contamination - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>mCCDA</subject><subject>Meat - microbiology</subject><subject>Microbiology</subject><subject>Sensitivity and Specificity</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp90EFrFDEUB_Agil2rn0CQXMTTjHmZZidz8CBLW4WCF3ssIZO8aJbMZEwyC_vtTd213szlT-D3HsmfkLfAWmCw_bhvJz9haTkDaIG3NZ6RDcieN7ITw3Oyqapvegb8grzKec8YiO5KviQXvJ5tL7oNebg-6LDq4uNMo6NzPGCg-odOmbqYaPmJFOd1wvREdnpajiGO2hRMNC9LS_1Ml7iGko40YdE-0FxRwPyavHA6ZHxzzktyf3P9ffeluft2-3X3-a4xnZSlsf0WpRHSoQBhZb0y45yw1sLQOX4luRYW6mfkKO0AIIUbpTAj6gE0Y7K7JB9Oe5cUf62Yi5p8NhiCnjGuWQ0wcC4G6KvsTtKkmHNCp5bkJ52OCph6rFXt1Z9a1WOtCriqUafenfev44T2aeZvjxW8PwOdjQ4u6dn4_M8JwSVn2-o-nRzWNg4ek8rG42zQ-oSmKBv9fx_yGyp_l8I</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Ahmed, R.</creator><creator>León-Velarde, C.G.</creator><creator>Odumeru, J.A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120201</creationdate><title>Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples</title><author>Ahmed, R. ; León-Velarde, C.G. ; Odumeru, J.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-d76e8c58fe515d8d760cff5ddd193f2482a5d11678b8d91185fb85cbea91a0083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agar - chemistry</topic><topic>Animals</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>BCCA</topic><topic>Biological and medical sciences</topic><topic>Campylobacter</topic><topic>Campylobacter - isolation & purification</topic><topic>CASA</topic><topic>CCA</topic><topic>CFA</topic><topic>Chickens</topic><topic>Colony Count, Microbial - methods</topic><topic>Culture Media - chemistry</topic><topic>Food Contamination - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>mCCDA</topic><topic>Meat - microbiology</topic><topic>Microbiology</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahmed, R.</creatorcontrib><creatorcontrib>León-Velarde, C.G.</creatorcontrib><creatorcontrib>Odumeru, J.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahmed, R.</au><au>León-Velarde, C.G.</au><au>Odumeru, J.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>88</volume><issue>2</issue><spage>304</spage><epage>310</epage><pages>304-310</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>The purpose of this study was to examine the performance of novel agars for the identification and enumeration of Campylobacter species. The analytical sensitivity and specificity of Campylobacter Selective agar (CASA), Brilliance CampyCount agar (BCCA) and CampyFoodIDagar (CFA) for 84 Campylobacter spp. isolates and 50 non-Campylobacter spp. isolates from 37 distinct genera were of 100% sensitivity, with a 98% specificity for BCCA and CFA, and a 100% specificity for CASA. The application of these selective agars for Campylobacter spp. enumeration in comparison to the conventional agars, modified charcoal cefoperazonedeoxycholate agar (mCCDA) and Campy-Cefex (CCA) was examined using Campylobacter jejuni and Campylobacter coli inoculated samples. From C. jejuni inoculated samples, recovery on BCCA was significantly greater than other media (p<0.05). Recovery on CASA was not significantly different from mCCDA and CCA (p>0.05). With C. coli inoculated samples, recovery was significantly greater on BCCA and CASA than with other media (p<0.05). The recovery of both C. jejuni and C. coli from inoculated samples with CFA was significantly less than with other media (P<0.05). CASA was able to effectively inhibit and differentiate Campylobacter spp. from background microflora while false positive organisms occurred with BCCA and CFA. An examination of 483 randomly selected suspect Campylobacter colonies from naturally contaminated samples demonstrated a colony confirmation rate for CCA, CFA, BCCA, mCCDA, and CASA, of 84%, 87%, 88%, 90%, and 100%, respectively. The media evaluated present an alternative to conventional selective agars for the identification and enumeration of thermotolerant Campylobacter spp. from samples of poultry origin through the farm to fork continuum.
► Assessed novel agars for the isolation and enumeration of Campylobacter spp. ► Agars were evaluated with the use of artificial and naturally contaminated samples. ► Two novel agars showed results comparable to conventional agars. ► False positive organisms found on both novel and conventional agars. ► CASA showed effective inhibition and differentiation of background flora.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22226753</pmid><doi>10.1016/j.mimet.2011.12.011</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2012-02, Vol.88 (2), p.304-310 |
| issn | 0167-7012 1872-8359 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_919225917 |
| source | ScienceDirect Freedom Collection |
| subjects | Agar - chemistry Animals Bacteriological methods and techniques used in bacteriology Bacteriology BCCA Biological and medical sciences Campylobacter Campylobacter - isolation & purification CASA CCA CFA Chickens Colony Count, Microbial - methods Culture Media - chemistry Food Contamination - analysis Fundamental and applied biological sciences. Psychology mCCDA Meat - microbiology Microbiology Sensitivity and Specificity |
| title | Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T14%3A34%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20novel%20agars%20for%20the%20enumeration%20of%20Campylobacter%20spp.%20in%20poultry%20retail%20samples&rft.jtitle=Journal%20of%20microbiological%20methods&rft.au=Ahmed,%20R.&rft.date=2012-02-01&rft.volume=88&rft.issue=2&rft.spage=304&rft.epage=310&rft.pages=304-310&rft.issn=0167-7012&rft.eissn=1872-8359&rft.coden=JMIMDQ&rft_id=info:doi/10.1016/j.mimet.2011.12.011&rft_dat=%3Cproquest_cross%3E919225917%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c388t-d76e8c58fe515d8d760cff5ddd193f2482a5d11678b8d91185fb85cbea91a0083%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=919225917&rft_id=info:pmid/22226753&rfr_iscdi=true |