Validation of a Gas Chromatography-Ion Trap-Tandem Mass Spectrometry Assay for the Simultaneous Quantification of Cocaine, Benzoylecgonine, Cocaethylene, Morphine, Codeine, and 6-Acetylmorphine in Aqueous Solution, Blood, and Skeletal Muscle Tissue

A gas chromatography-ion trap-tandem mass spectrometry method was developed and validated for the simultaneous solid-phase extraction and quantification of cocaine, benzoylecgonine, cocaethylene, morphine, codeine, and 6-monoacteylmorphine in blood, muscle tissue, and water. An assay for total morph...

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Bibliographic Details
Published in:Journal of analytical toxicology 2012-01, Vol.36 (1), p.1-11
Main Authors: Rees, Kelly A., McLaughlin, Poppy A., Osselton, M. David
Format: Article
Language:English
Subjects:
Animals
Blood
Cocaethylene
Cocaine
Cocaine - analogs & derivatives
Cocaine - analysis
Codeine
Codeine - analysis
Deer
Gas Chromatography-Mass Spectrometry - methods
Horses
Hydrolysis
Mass spectroscopy
Metabolites
Morphine
Morphine - analysis
Morphine Derivatives - analysis
Muscle, Skeletal - chemistry
Opiates
Skeletal muscle
Solutions
Tandem Mass Spectrometry - methods
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Summary:A gas chromatography-ion trap-tandem mass spectrometry method was developed and validated for the simultaneous solid-phase extraction and quantification of cocaine, benzoylecgonine, cocaethylene, morphine, codeine, and 6-monoacteylmorphine in blood, muscle tissue, and water. An assay for total morphine in blood and muscle was also validated. The limit of quantification was ≤ 0.01 mg/kg in muscle and ≤ 0.005 mg/L in blood and water. Good linearity was observed over the concentration ranges studied (r ≥ 0.99). The repeatability (% RSD) at the three concentration levels was typically ≤ 15% and never exceeded 17%. Intermediate precision of ≤ 16% was obtained for all matrices. Deviation < 20% of the nominal concentration was obtained in all repeatability and intermediate precision experiments. Extraction recoveries for cocaine and metabolites ranged from 91% to 110% in water, 81% to 110% in blood, and 61% to 75% in muscle. Recoveries for opiates ranged from 59% to 104% in water, 50% to 95% in blood, and 41% to 79% in muscle. The hydrolysis efficiency for the total morphine assay in blood and muscle ranged from 91% to 99% with within-day and intermediate precisions of ≤ 14% and ≤ 12%, respectively.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/bkr006