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Biomarker-Mediated Disruption of Coffee-Ring Formation as a Low Resource Diagnostic Indicator
The ring pattern resulting from the unique microfluidics in an evaporating coffee drop is a well-studied mass transport phenomenon generating interest in the research community mostly from a mechanistic perspective. In this report, we describe how biomarker-induced particle–particle assemblies, magn...
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Published in: | Langmuir 2012-01, Vol.28 (4), p.2187-2193 |
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creator | Trantum, Joshua R Wright, David W Haselton, Frederick R |
description | The ring pattern resulting from the unique microfluidics in an evaporating coffee drop is a well-studied mass transport phenomenon generating interest in the research community mostly from a mechanistic perspective. In this report, we describe how biomarker-induced particle–particle assemblies, magnetic separation, and evaporation-driven ring formation can be combined for simple pathogen detection. In this assay design, the presence of biomarkers causes self-assembly of a magnetic nanoparticle and a fluorescently labeled micrometer-sized particle. A small spherical magnet under the center of the drop prevents these assemblies from migrating to the drop’s edge while a nonreactive control particle flows to the edge forming a ring pattern. Thus the presence or absence of biomarker results in distinctly different distributions of particles in the dried drop. Proof-of-principle studies using poly-l-histidine, a peptide mimic of the malaria biomarker pfHRPII, show that the predicted particle distributions occur with a limit of detection of approximately 200–300 nM. |
doi_str_mv | 10.1021/la203903a |
format | article |
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Proof-of-principle studies using poly-l-histidine, a peptide mimic of the malaria biomarker pfHRPII, show that the predicted particle distributions occur with a limit of detection of approximately 200–300 nM.</description><subject>Antigens, Protozoan - metabolism</subject><subject>Biomarkers - analysis</subject><subject>Biomarkers - chemistry</subject><subject>Biomimetic Materials - chemistry</subject><subject>Biomimetic Materials - metabolism</subject><subject>Clinical Chemistry Tests - instrumentation</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Histidine - chemistry</subject><subject>Histidine - metabolism</subject><subject>Limit of Detection</subject><subject>Magnets - chemistry</subject><subject>Malaria - diagnosis</subject><subject>Malaria - metabolism</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Nanoparticles - chemistry</subject><subject>Protozoan Proteins - metabolism</subject><subject>Volatilization</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kE0vBEEQhjtCWMvBH5C5CA5D9cfMdB9Zn8mKRDjKpLa3Rprd6dU9E_HvNYuTOFVV3qfew8PYDocjDoIfz1CANCBxhQ14ISAvtKhW2QAqJfNKlXKDbcb4DABGKrPONoTgSuuiGLDHU-fnGF4o5Dc0ddjRNDtzMfSLzvk280028k1DlN-59im78GGOXwHGDLOxf8vuKPo-WEpf-NT62DmbXbdTZ7HzYYutNTiLtP09h-zh4vx-dJWPby-vRyfjHBWXXS6MqMhgUQnVmBIKsNSgBjXRUkNZCp0OKSAtUtkJamukQAuinBjiUJIcsv1l7yL4155iV89dtDSbYUu-j7XhRoiq4jyRB_-SSZ9SvNJcJPRwidrgYwzU1Ivgkqv3mkP96b3-9Z7Y3e_afjKn6S_5IzoBe0sAbayfk7E2-fij6APyD4dZ</recordid><startdate>20120131</startdate><enddate>20120131</enddate><creator>Trantum, Joshua R</creator><creator>Wright, David W</creator><creator>Haselton, Frederick R</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20120131</creationdate><title>Biomarker-Mediated Disruption of Coffee-Ring Formation as a Low Resource Diagnostic Indicator</title><author>Trantum, Joshua R ; 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subjects | Antigens, Protozoan - metabolism Biomarkers - analysis Biomarkers - chemistry Biomimetic Materials - chemistry Biomimetic Materials - metabolism Clinical Chemistry Tests - instrumentation Fluorescent Dyes - chemistry Histidine - chemistry Histidine - metabolism Limit of Detection Magnets - chemistry Malaria - diagnosis Malaria - metabolism Microfluidic Analytical Techniques - instrumentation Nanoparticles - chemistry Protozoan Proteins - metabolism Volatilization |
title | Biomarker-Mediated Disruption of Coffee-Ring Formation as a Low Resource Diagnostic Indicator |
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