Improvement of frequency sensitivity of series photodetector frequency circuit system and its application for Cy5 fluorescence detection

• Published in: Optik (Stuttgart) 2011-10, Vol.122 (19), p.1747-1752
• Main Authors: Hsu, Yuh Ming, Chang, Chung Cheng
• Format: Article
• Language: English
• Subjects: Adjustment; Bias; Capacitance; Circuits; Cy5; DNA probe; Dyes; Fluorescence; Fluorescence dye concentration; Photodetector; Photodetectors; Sensors; Series photodetector frequency circuit system
• ISSN: 0030-4026; 1618-1336
• DOI: 10.1016/j.ijleo.2010.10.036

This study elucidated the frequency characteristics of series photodetector frequency circuit system for detection of DNA probe ET996 marked with fluorescence dye Cy5. We developed 48 MHz series photodetector frequency circuit system with good sensitivity for fluorescence detection. In accordance with the theory of series photodetector frequency circuit system, the frequency sensitivity can be improved by adjusting circuit parameters such as A (tan θ), C q , C 0, and C p . In this research of A adjustment, the capacitance parameter C m of 48 MHz series photodetector frequency circuit system was adjusted to improve the frequency sensitivity for detection of fluorescence dye concentration; moreover, the bias of photodetector was also adjusted to improve the frequency sensitivity. In the optimal conditions of capacitance match and photodetector bias, the detection limit of ET996-Cy5 fluorescence dye concentration 2 pmol/L can be measured by 48 MHz sensor system. The results of fluorescence experiment also demonstrated that the frequency shift of 48 MHz sensor system was linearly related to the logarithm of fluorescence dye concentration from 200 nmol/L to 2 pmol/L. The frequency method can be applied simply and the detection limit of ET996-Cy5 fluorescence dye concentration was lower than the conventional fluorescence technique by 2 orders.