Oleaginous yeast Cryptococcus curvatus culture with dark fermentation hydrogen production effluent as feedstock for microbial lipid production

• Published in: International journal of hydrogen energy 2011-08, Vol.36 (16), p.9542-9550
• Main Authors: Chi, Zhanyou, Zheng, Yubin, Ma, Jingwei, Chen, Shulin
• Format: Article
• Language: English
• Subjects: Alternative fuels. Production and utilization; Applied sciences; Biodiesel; Biohydrogen; Carbon; Cryptococcus curvatus; Culture; Energy; Exact sciences and technology; Feedstock; Fuels; Hydrogen; Hydrogen production; Inhibitors; Lipids; Oleaginous yeast; Organic waste; Wastes; Yeast
• ISSN: 0360-3199; 1879-3487
• DOI: 10.1016/j.ijhydene.2011.04.124

Volatile fatty acids (VFA) from dark fermentation hydrogen production were tested as carbon sources for the culture of oleaginous yeast Cryptococcus curvatus, which is a promising feedstock for biofuel production. The optimal acetate concentration and pH were investigated when potassium acetate was used as the sole carbon source. Comparisons were then made when hydrogen production effluent (HPE) from synthetic wastewater was tested as feedstock. A pH-stat culture fed with acetic acid ultimately produced 168 g/L biomass, with a lipid content of 75.0%. No inhibitor to yeast growth was produced in the hydrogen production process. However, inhibition occurred in culture with HPE from food waste (FW), indicating that inhibitors may be present in the original raw food waste. This inhibition could be avoided by a process that uses glucose as the initial carbon source and then is continuously fed with FW-HPE. The biomass productivity in this continuous culture process reached 0.34 g/L/h, but the lipid content was only 13.5%. These results suggest that FW-HPE alone is not an optimal feedstock, but HPE derived from nitrogen-deficient waste streams could be good feedstocks. This study provides preliminary evidence for the feasibility of using organic waste for the co-production of hydrogen and lipid. ► Preliminary evidence for integrating lipid production with hydrogen production. ► pH and acetate concentration optimized for C. curvatus' growth with acetate as feedstock. ► VFA produced in biohydrogen production used as feedstock to grow oleaginous yeast. ► No inhibitor to C. curvatus' growth produced in dark fermentation hydrogen production. ► Inhibitors in food waste can be avoided by using continuous culture strategy.