Pathway analysis of gene expression in local lymph nodes draining skin exposed to three different sensitizers

• Published in: Journal of applied toxicology 2011-07, Vol.31 (5), p.455-462
• Main Authors: Ku, Hyun-Ok, Jeong, Sang-Hee, Kang, Hwan-Goo, Son, Seong-Wan, Yun, So-mi, Ryu, Doug-Young
• Format: Article
• Language: English
• Subjects: 1-chloro-2; 1‐chloro‐2,4‐dinitrobenzene; 2-phenyl-4-ethoxymethylene-5-oxazolone; 4-diisocyanate; 4-dinitrobenzene; Administration, Topical; Allergens - toxicity; Animals; Biological and medical sciences; Chemical and industrial products toxicology. Toxic occupational diseases; Croton Oil - toxicity; Dinitrochlorobenzene - toxicity; gene expression; Gene Expression - drug effects; Genomics; Local Lymph Node Assay; Lymph Nodes - drug effects; Lymph Nodes - metabolism; Lymph Nodes - pathology; Medical sciences; Mice; Oligonucleotide Array Sequence Analysis; Oxazolone - toxicity; pathway; Reverse Transcriptase Polymerase Chain Reaction; skin sensitization; Solvents; toluene 2; toluene 2, 4‐diisocyanate; Toluene 2,4-Diisocyanate - toxicity; Toxicology
• ISSN: 0260-437X; 1099-1263; 1099-1263
• DOI: 10.1002/jat.1646

ABSTRACT Genomic analysis in the local lymph node assays (LLNAs) is useful for assessing skin sensitization of chemicals and providing insights into mechanisms of sensitization. In this study, we collected 1406 genes from previous microarray findings, validated changes in their expression by RT‐PCR analysis in local lymph nodes draining skin exposed to different sensitizers, and interpreted their biological function through pathway‐based genomic analysis, in which 468 genes were identified as being in the KEGG pathway database. The top‐ranked functions (P