Stimulated Raman hyperspectral imaging based on spectral filtering of broadband fiber laser pulses

We demonstrate a technique of hyperspectral imaging in stimulated Raman scattering (SRS) microscopy using a tunable optical filter, whose transmission wavelength can be varied quickly by a galvanometer mirror. Experimentally, broadband Yb fiber laser pulses are synchronized with picosecond Ti:sapphi...

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Bibliographic Details
Published in:Optics letters 2012-02, Vol.37 (3), p.431-433
Main Authors: Ozeki, Yasuyuki, Umemura, Wataru, Sumimura, Kazuhiko, Nishizawa, Norihiko, Fukui, Kiichi, Itoh, Kazuyoshi
Format: Article
Language:English
Subjects:
Beads
Broadband
Fiber lasers
Filtering
Hyperspectral imaging
Lasers
Microscopy - instrumentation
Narrowband
Optical Fibers
Spectra
Spectrum Analysis, Raman - instrumentation
Wavelengths
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Summary:We demonstrate a technique of hyperspectral imaging in stimulated Raman scattering (SRS) microscopy using a tunable optical filter, whose transmission wavelength can be varied quickly by a galvanometer mirror. Experimentally, broadband Yb fiber laser pulses are synchronized with picosecond Ti:sapphire pulses, and then spectrally filtered out by the filter. After amplification by fiber amplifiers, we obtain narrowband pulses with a spectral width of 225 cm(-1). By using these pulses, we accomplish SRS imaging of polymer beads with spectral information.
ISSN:0146-9592
1539-4794
DOI:10.1364/ol.37.000431