Validation of an LC Method to Determine Skin Retention Profile of Genistein from Nanoemulsions Incorporated in Hydrogels

Recent studies have shown the effect of soy isoflavones in preventing skin photoaging and photocarcinogenesis, especially for genistein (GEN). Nanoemulsions have been proposed as a delivery system for GEN administration due to the low water solubility of this isoflavone. This article describes the v...

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Bibliographic Details
Published in:Journal of chromatographic science 2012-02, Vol.50 (2), p.114-118
Main Authors: de Vargas, Bethânia Andrade, Argenta, Débora Fretes, Borghetti, Greice, Koester, Leticia Scherer, Bassani, Valquiria Linck, Teixeira, Helder Ferreira
Format: Article
Language:English
Subjects:
Analysis
Animals
Applied sciences
Biological and medical sciences
Chemical industry and chemicals
Chromatography, Liquid - methods
Cosmetics, toiletries
Emulsions
Exact sciences and technology
General pharmacology
Genistein - metabolism
Hydrogels
Medical sciences
Nanostructures
Pharmacology. Drug treatments
Reproducibility of Results
Skin - metabolism
Spectrophotometry, Ultraviolet
Swine
Washing products. Cosmetics and toiletries. Perfumes
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Summary:Recent studies have shown the effect of soy isoflavones in preventing skin photoaging and photocarcinogenesis, especially for genistein (GEN). Nanoemulsions have been proposed as a delivery system for GEN administration due to the low water solubility of this isoflavone. This article describes the validation of an isocratic liquid chromatography method to determine GEN in porcine ear skin layers from nanoemulsions before and after incorporation into hydrogels. The analyses are performed on a reversed-phase C18 column using a mobile phase composed of methanol-water (70:30, v/v) under acid conditions (at pH 3.0) and UV detection at 270 nm. The method is linear in the range of 0.1-10 µg/mL (r2 > 0.999) in the presence of skin extracts. The low limit of quantitation is estimated as 0.1 µg/mL. No interferences from formulation excipients or skin layer compounds are detected. The RSD values for intra- and inter-day precision are lower than 15%. Recovery ranged from approximately 90% to 110%. The method is applied to estimate GEN retention in the skin from formulations using Franz diffusion cells. The highest amount of GEN is detected in the epidermis (185 µg/cm2). In conclusion, the method proved to be specific, precise, and accurate in determining GEN amounts from formulations in skin retention studies.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmr038