Effect of ultraviolet filters on skin superoxide dismutase activity in hairless mice after a single dose of ultraviolet radiation

• Published in: European journal of pharmaceutics and biopharmaceutics 2012-02, Vol.80 (2), p.387-392
• Main Authors: Vilela, Fernanda Maria Pinto, Fonseca, Yris Maria, Jabor, José Roberto, Vicentini, Fabiana T.M.C., Fonseca, Maria José Vieira
• Format: Article
• Language: English
• Subjects: Administration, Cutaneous; Animals; Benzophenone-3; Benzophenones - administration & dosage; Benzophenones - pharmacology; Biological and medical sciences; Cinnamates - administration & dosage; Cinnamates - pharmacology; Cytochromes c - metabolism; Drug Combinations; Female; General pharmacology; Male; Medical sciences; Mice; Mice, Hairless; Octyl methoxycinnamate; Octyl salicylate; Permeability; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Photodegradation; Salicylates - administration & dosage; Salicylates - pharmacology; Skin - drug effects; Skin - radiation effects; Spectrophotometry; Sunscreening Agents - administration & dosage; Sunscreening Agents - pharmacology; Superoxide dismutase; Superoxide Dismutase - antagonists & inhibitors; Superoxide Dismutase - metabolism; Ultraviolet Rays - adverse effects; UV radiation
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2011.10.005

Effect of a cream gel formulation containing the UV filters benzophenone-3, octyl methoxycinnamate, and octyl salicylate on skin superoxide dismutase (SOD) after a single dose of UVR (2.87J/cm2). Organic sunscreens may decrease their protective capability and also behave as photo-oxidants upon ultraviolet radiation (UVR) exposure. The present study investigated the effect of a cream gel formulation containing the UV filters benzophenone-3, octyl methoxycinnamate, and octyl salicylate on skin superoxide dismutase (SOD) after a single dose of UVR (2.87J/cm2). The retention of these UV filters was first evaluated in vivo using hairless mice to guarantee the presence of the filters in the skin layers at the moment of irradiation. The in vivo effect of the UV filters on skin SOD was then assayed spectrophotometrically via the reduction of cytochrome c. The cream gel formulation promoted the penetration of the three UV filters into the epidermis and the dermis at one hour post-application. A significant decrease in SOD activity was observed in irradiated animals treated with sunscreen formulation. However, no effect on SOD activity in skin was observed by the isolated presence of the sunscreens, the formulation components, or the exposure to UVR. The sunscreens may have formed degradation products under UVR that may have either inhibited the enzyme or generated reactive species in the skin.