Simultaneous Purification and Site-Specific Modification of Pyrroline-Carboxy-Lysine Proteins

Sticky residue: Pyrroline‐carboxy‐lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site‐specifically modified with functional probes du...

Full description

Saved in:
Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2012-02, Vol.13 (3), p.364-366
Main Authors: Chiu, Hsien-Po, Grünewald, Jan, Hao, Xueshi, Brock, Ansgar, Okach, Linda, Uno, Tetsuo, Geierstanger, Bernhard H.
Format: Article
Language:English
Subjects:
affinity tags
Benzaldehydes - chemistry
Binding Sites
Lysine - analogs & derivatives
Lysine - chemistry
Lysine - metabolism
Molecular Structure
protein engineering
protein expression
protein purification
Proteins - chemistry
Proteins - isolation & purification
pyrroline-carboxy-lysine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sticky residue: Pyrroline‐carboxy‐lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site‐specifically modified with functional probes during the elution process (see scheme).
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201100684