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Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay
To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphor...
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| Published in: | Analytical biochemistry 2012-02, Vol.421 (2), p.773-775 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c439t-b6162f621909121a25f726df76c324911cc0a2e4748461d247593426339919003 |
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| cites | cdi_FETCH-LOGICAL-c439t-b6162f621909121a25f726df76c324911cc0a2e4748461d247593426339919003 |
| container_end_page | 775 |
| container_issue | 2 |
| container_start_page | 773 |
| container_title | Analytical biochemistry |
| container_volume | 421 |
| creator | Tsuchiya, Akira Asai, Daisuke Kang, Jeong-Hun Mori, Takeshi Niidome, Takuro Katayama, Yoshiki |
| description | To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI–TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays. |
| doi_str_mv | 10.1016/j.ab.2011.08.035 |
| format | article |
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The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. 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The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI–TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.</description><subject>amino acids</subject><subject>correlation</subject><subject>Correlation coefficient</subject><subject>desorption</subject><subject>ionization</subject><subject>MALDI–TOF MS</subject><subject>matrix-assisted laser desorption-ionization mass spectrometry</subject><subject>monitoring</subject><subject>peptides</subject><subject>Peptides - chemistry</subject><subject>Phosphorylation</subject><subject>Protein kinase</subject><subject>Radioactivity</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kT2P1DAQhi0E4paDngrSUSXnrzgxHVrxJZ1EAVdbjjO58yqJF4_3IPwmfiQOWaCiGI01et535HkJec5oxShTV4fKdhWnjFW0raioH5Ado1qVVFD9kOwopaLkSjcX5AnigWZQ1uoxueBM81q2Ykd-7kOMMNrkw1x0kL4BzMXxLmCuuJzncW1YdEsx2RT999IiekzQF6NFiEUPGOJxRa9y-R-bKvkJyjCUw-hv71KWIhZ4BJdimCDFpbCzHZdslB993tH7YF3y9z55-L3s3wiKLLbLU_JosCPCs3O_JDfv3n7ZfyivP73_uH9zXTopdCo7xRQfVP4k1Ywzy-uh4aofGuUEl5ox56jlIBvZSsV6LptaC8mVEFpnDRWX5NXme4zh6wkwmcmjg3G0M4QTGs2pULVueSbpRroYECMM5hj9ZONiGDVrROZgbGfWiAxtTY4oS16czU_dBP1fwZ9MMvByAwYbjL2NHs3N5-ygcpysZXIlXm8E5CPce4gGnYfZQe9jvq_pg____l_twq2_</recordid><startdate>20120215</startdate><enddate>20120215</enddate><creator>Tsuchiya, Akira</creator><creator>Asai, Daisuke</creator><creator>Kang, Jeong-Hun</creator><creator>Mori, Takeshi</creator><creator>Niidome, Takuro</creator><creator>Katayama, Yoshiki</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120215</creationdate><title>Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay</title><author>Tsuchiya, Akira ; Asai, Daisuke ; Kang, Jeong-Hun ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-b6162f621909121a25f726df76c324911cc0a2e4748461d247593426339919003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>amino acids</topic><topic>correlation</topic><topic>Correlation coefficient</topic><topic>desorption</topic><topic>ionization</topic><topic>MALDI–TOF MS</topic><topic>matrix-assisted laser desorption-ionization mass spectrometry</topic><topic>monitoring</topic><topic>peptides</topic><topic>Peptides - chemistry</topic><topic>Phosphorylation</topic><topic>Protein kinase</topic><topic>Radioactivity</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsuchiya, Akira</creatorcontrib><creatorcontrib>Asai, Daisuke</creatorcontrib><creatorcontrib>Kang, Jeong-Hun</creatorcontrib><creatorcontrib>Mori, Takeshi</creatorcontrib><creatorcontrib>Niidome, Takuro</creatorcontrib><creatorcontrib>Katayama, Yoshiki</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsuchiya, Akira</au><au>Asai, Daisuke</au><au>Kang, Jeong-Hun</au><au>Mori, Takeshi</au><au>Niidome, Takuro</au><au>Katayama, Yoshiki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2012-02-15</date><risdate>2012</risdate><volume>421</volume><issue>2</issue><spage>773</spage><epage>775</epage><pages>773-775</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF MS) analysis, we prepared 136 peptide substrates. 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| ispartof | Analytical biochemistry, 2012-02, Vol.421 (2), p.773-775 |
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| language | eng |
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| subjects | amino acids correlation Correlation coefficient desorption ionization MALDI–TOF MS matrix-assisted laser desorption-ionization mass spectrometry monitoring peptides Peptides - chemistry Phosphorylation Protein kinase Radioactivity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods |
| title | Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay |
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