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Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)-γ-Treated Decidual Cells
Citation Borbely AU, Fontenele‐Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)‐γ‐treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83 Problem In this study, we explored the relatio...
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| Published in: | American journal of reproductive immunology (1989) 2012-01, Vol.67 (1), p.73-83 |
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| container_end_page | 83 |
| container_issue | 1 |
| container_start_page | 73 |
| container_title | American journal of reproductive immunology (1989) |
| container_volume | 67 |
| creator | Borbely, Alexandre U. Fontenele-Neto, José D. Vidsiunas, Alex K. Gomes, Sara Z. Hoshida, Mara S. de Oliveira, Sérgio F. Bevilacqua, Estela |
| description | Citation Borbely AU, Fontenele‐Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)‐γ‐treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83
Problem In this study, we explored the relationship between decidual cells (DC) and interferon (IFN)‐γ, in the presence or absence of ectoplacental cone (EC) using a coculture system.
Method of study Decidual cells and EC were isolated from pregnant mice on gestation day 7.5. DCs were cultured for 48 hr and then treated with fresh EC. After characterization, they were treated with IFN‐γ, and cell death was evaluated.
Results Interferon‐γ drastically increased decidual apoptosis, which was partially reverted by the addition of EC to the IFN‐γ‐treated decidual culture. Moreover, the addition of EC to non‐treated DC cultures was also capable of attenuating death rates.
Conclusion Resistance to apoptosis may be induced in DC by the EC. This suggests that EC may participate in the inhibition of IFN‐γ‐dependent apoptosis and, therefore, play important role for DC survival in a cytokine‐enriched placental environment. |
| doi_str_mv | 10.1111/j.1600-0897.2011.01060.x |
| format | article |
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Problem In this study, we explored the relationship between decidual cells (DC) and interferon (IFN)‐γ, in the presence or absence of ectoplacental cone (EC) using a coculture system.
Method of study Decidual cells and EC were isolated from pregnant mice on gestation day 7.5. DCs were cultured for 48 hr and then treated with fresh EC. After characterization, they were treated with IFN‐γ, and cell death was evaluated.
Results Interferon‐γ drastically increased decidual apoptosis, which was partially reverted by the addition of EC to the IFN‐γ‐treated decidual culture. Moreover, the addition of EC to non‐treated DC cultures was also capable of attenuating death rates.
Conclusion Resistance to apoptosis may be induced in DC by the EC. This suggests that EC may participate in the inhibition of IFN‐γ‐dependent apoptosis and, therefore, play important role for DC survival in a cytokine‐enriched placental environment.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.2011.01060.x</identifier><identifier>PMID: 21883617</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - immunology ; Cell Communication - immunology ; Cell culture ; Cell death ; Cell survival ; Cell Survival - drug effects ; Cell Survival - immunology ; coculture assay ; Coculture Techniques ; decidua ; Decidua - cytology ; Decidua - drug effects ; Decidua - immunology ; Decidua - metabolism ; Dendritic cells ; Diffusion Chambers, Culture ; Female ; gamma -Interferon ; Gestation ; Humans ; In Situ Nick-End Labeling ; Interferon-gamma - adverse effects ; Interferon-gamma - immunology ; interferon-γ ; mesometrial decidual cells ; Mice ; Microscopy, Fluorescence ; Organ Specificity - immunology ; Placenta ; Placenta - cytology ; Placenta - immunology ; Pregnancy ; Primary Cell Culture ; trophoblast ; TUNEL reaction</subject><ispartof>American journal of reproductive immunology (1989), 2012-01, Vol.67 (1), p.73-83</ispartof><rights>2011 John Wiley & Sons A/S</rights><rights>2011 John Wiley & Sons A/S.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4380-8b362dc5f0bb7ed2483e926ff822c1b4511aab1f065b5d056d8a27f71c35c4e33</citedby><cites>FETCH-LOGICAL-c4380-8b362dc5f0bb7ed2483e926ff822c1b4511aab1f065b5d056d8a27f71c35c4e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21883617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borbely, Alexandre U.</creatorcontrib><creatorcontrib>Fontenele-Neto, José D.</creatorcontrib><creatorcontrib>Vidsiunas, Alex K.</creatorcontrib><creatorcontrib>Gomes, Sara Z.</creatorcontrib><creatorcontrib>Hoshida, Mara S.</creatorcontrib><creatorcontrib>de Oliveira, Sérgio F.</creatorcontrib><creatorcontrib>Bevilacqua, Estela</creatorcontrib><title>Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)-γ-Treated Decidual Cells</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Citation Borbely AU, Fontenele‐Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)‐γ‐treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83
Problem In this study, we explored the relationship between decidual cells (DC) and interferon (IFN)‐γ, in the presence or absence of ectoplacental cone (EC) using a coculture system.
Method of study Decidual cells and EC were isolated from pregnant mice on gestation day 7.5. DCs were cultured for 48 hr and then treated with fresh EC. After characterization, they were treated with IFN‐γ, and cell death was evaluated.
Results Interferon‐γ drastically increased decidual apoptosis, which was partially reverted by the addition of EC to the IFN‐γ‐treated decidual culture. Moreover, the addition of EC to non‐treated DC cultures was also capable of attenuating death rates.
Conclusion Resistance to apoptosis may be induced in DC by the EC. This suggests that EC may participate in the inhibition of IFN‐γ‐dependent apoptosis and, therefore, play important role for DC survival in a cytokine‐enriched placental environment.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - immunology</subject><subject>Cell Communication - immunology</subject><subject>Cell culture</subject><subject>Cell death</subject><subject>Cell survival</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - immunology</subject><subject>coculture assay</subject><subject>Coculture Techniques</subject><subject>decidua</subject><subject>Decidua - cytology</subject><subject>Decidua - drug effects</subject><subject>Decidua - immunology</subject><subject>Decidua - metabolism</subject><subject>Dendritic cells</subject><subject>Diffusion Chambers, Culture</subject><subject>Female</subject><subject>gamma -Interferon</subject><subject>Gestation</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Interferon-gamma - adverse effects</subject><subject>Interferon-gamma - immunology</subject><subject>interferon-γ</subject><subject>mesometrial decidual cells</subject><subject>Mice</subject><subject>Microscopy, Fluorescence</subject><subject>Organ Specificity - immunology</subject><subject>Placenta</subject><subject>Placenta - cytology</subject><subject>Placenta - immunology</subject><subject>Pregnancy</subject><subject>Primary Cell Culture</subject><subject>trophoblast</subject><subject>TUNEL reaction</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAUhS0EoqXwCsg7YJFwHcc_WbAYTf-GliJVpV1ajnMDGTJxGidi-ly8B8-Ew5TZgje-uj7fudY9hFAGKYvn_TplEiABXag0A8ZSYCAh3T4hh_uHp7GGXCYqB31AXoSwBoh9rp6Tg4xpzSVTh6Q7caPvW-uwG21Ll75DuuqqyWGg1xiaMNrOIR09XfS-H33s0Kaj583Xb_TYh6jydQRGHGocfEffrk6v3iW_fiY3A9oRK3qMrqmm2RrbNrwkz2rbBnz1eB-RL6cnN8vz5PLz2Wq5uExczjUkuuQyq5yooSwVVlmuORaZrGudZY6VuWDM2pLVIEUpKhCy0jZTtWKOC5cj50fkzc63H_z9hGE0mya4-APboZ-CKTJQBcQN_FvJWMFBCxGVeqd0gw9hwNr0Q7Oxw4NhYOZYzNrM2zfz9s0ci_kTi9lG9PXjkKncYLUH_-YQBR92gh9Niw__bWwWH1dzFflkx8fAcLvn7fDdSMWVMHdXZ-bi4g4-3d5Kc81_A49KqlE</recordid><startdate>201201</startdate><enddate>201201</enddate><creator>Borbely, Alexandre U.</creator><creator>Fontenele-Neto, José D.</creator><creator>Vidsiunas, Alex K.</creator><creator>Gomes, Sara Z.</creator><creator>Hoshida, Mara S.</creator><creator>de Oliveira, Sérgio F.</creator><creator>Bevilacqua, Estela</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201201</creationdate><title>Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)-γ-Treated Decidual Cells</title><author>Borbely, Alexandre U. ; Fontenele-Neto, José D. ; Vidsiunas, Alex K. ; Gomes, Sara Z. ; Hoshida, Mara S. ; de Oliveira, Sérgio F. ; Bevilacqua, Estela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4380-8b362dc5f0bb7ed2483e926ff822c1b4511aab1f065b5d056d8a27f71c35c4e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - immunology</topic><topic>Cell Communication - immunology</topic><topic>Cell culture</topic><topic>Cell death</topic><topic>Cell survival</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - immunology</topic><topic>coculture assay</topic><topic>Coculture Techniques</topic><topic>decidua</topic><topic>Decidua - cytology</topic><topic>Decidua - drug effects</topic><topic>Decidua - immunology</topic><topic>Decidua - metabolism</topic><topic>Dendritic cells</topic><topic>Diffusion Chambers, Culture</topic><topic>Female</topic><topic>gamma -Interferon</topic><topic>Gestation</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>Interferon-gamma - adverse effects</topic><topic>Interferon-gamma - immunology</topic><topic>interferon-γ</topic><topic>mesometrial decidual cells</topic><topic>Mice</topic><topic>Microscopy, Fluorescence</topic><topic>Organ Specificity - immunology</topic><topic>Placenta</topic><topic>Placenta - cytology</topic><topic>Placenta - immunology</topic><topic>Pregnancy</topic><topic>Primary Cell Culture</topic><topic>trophoblast</topic><topic>TUNEL reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borbely, Alexandre U.</creatorcontrib><creatorcontrib>Fontenele-Neto, José D.</creatorcontrib><creatorcontrib>Vidsiunas, Alex K.</creatorcontrib><creatorcontrib>Gomes, Sara Z.</creatorcontrib><creatorcontrib>Hoshida, Mara S.</creatorcontrib><creatorcontrib>de Oliveira, Sérgio F.</creatorcontrib><creatorcontrib>Bevilacqua, Estela</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borbely, Alexandre U.</au><au>Fontenele-Neto, José D.</au><au>Vidsiunas, Alex K.</au><au>Gomes, Sara Z.</au><au>Hoshida, Mara S.</au><au>de Oliveira, Sérgio F.</au><au>Bevilacqua, Estela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)-γ-Treated Decidual Cells</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2012-01</date><risdate>2012</risdate><volume>67</volume><issue>1</issue><spage>73</spage><epage>83</epage><pages>73-83</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Citation Borbely AU, Fontenele‐Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)‐γ‐treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83
Problem In this study, we explored the relationship between decidual cells (DC) and interferon (IFN)‐γ, in the presence or absence of ectoplacental cone (EC) using a coculture system.
Method of study Decidual cells and EC were isolated from pregnant mice on gestation day 7.5. DCs were cultured for 48 hr and then treated with fresh EC. After characterization, they were treated with IFN‐γ, and cell death was evaluated.
Results Interferon‐γ drastically increased decidual apoptosis, which was partially reverted by the addition of EC to the IFN‐γ‐treated decidual culture. Moreover, the addition of EC to non‐treated DC cultures was also capable of attenuating death rates.
Conclusion Resistance to apoptosis may be induced in DC by the EC. This suggests that EC may participate in the inhibition of IFN‐γ‐dependent apoptosis and, therefore, play important role for DC survival in a cytokine‐enriched placental environment.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21883617</pmid><doi>10.1111/j.1600-0897.2011.01060.x</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Apoptosis Apoptosis - drug effects Apoptosis - immunology Cell Communication - immunology Cell culture Cell death Cell survival Cell Survival - drug effects Cell Survival - immunology coculture assay Coculture Techniques decidua Decidua - cytology Decidua - drug effects Decidua - immunology Decidua - metabolism Dendritic cells Diffusion Chambers, Culture Female gamma -Interferon Gestation Humans In Situ Nick-End Labeling Interferon-gamma - adverse effects Interferon-gamma - immunology interferon-γ mesometrial decidual cells Mice Microscopy, Fluorescence Organ Specificity - immunology Placenta Placenta - cytology Placenta - immunology Pregnancy Primary Cell Culture trophoblast TUNEL reaction |
| title | Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)-γ-Treated Decidual Cells |
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