Methicillin-resistant Staphylococcus aureus vancomycin susceptibility testing: methodology correlations, temporal trends and clonal patterns

Objectives To determine the correlation between various vancomycin MIC testing methodologies and explore the phenomenon of MIC creep. Methods A total of 417 consecutive non-duplicate methicillin-resistant Staphylococcus aureus (MRSA) bloodstream isolates from Liverpool Hospital between 1997 and 2008...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2011-10, Vol.66 (10), p.2284-2287
Main Authors: van Hal, Sebastiaan J., Barbagiannakos, Thelma, Jones, Mark, Wehrhahn, Michael C., Mercer, Joanne, Chen, Dehua, Paterson, David L., Gosbell, Iain B.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial diseases
Biological and medical sciences
Correlation analysis
Drug resistance
Drug Resistance, Multiple, Bacterial
Human bacterial diseases
Humans
Infectious diseases
Medical sciences
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - isolation & purification
Microbial Sensitivity Tests - methods
Pharmacology. Drug treatments
Staphylococcal infections, streptococcal infections, pneumococcal infections
Staphylococcus aureus
Staphylococcus infections
Vancomycin - pharmacology
Vancomycin Resistance
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Summary:Objectives To determine the correlation between various vancomycin MIC testing methodologies and explore the phenomenon of MIC creep. Methods A total of 417 consecutive non-duplicate methicillin-resistant Staphylococcus aureus (MRSA) bloodstream isolates from Liverpool Hospital between 1997 and 2008 were retrieved. All isolates were classified using PFGE and underwent susceptibility testing for vancomycin using a standard Etest (AB bioMérieux, Solna, Sweden), Vitek2® (AST-P612; bioMérieux, Inc., Durham, NC, USA) and broth microdilution (BMD) performed as per the CLSI method. Results Over the 12 years, 78% (n = 326) of the isolates were multiresistant MRSA (ST239-like by PFGE, where ST stands for sequence type). The correlation between MIC testing methods was moderate with Spearman's correlation coefficients of 0.50 for BMD versus Etest (P 
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dkr280