Increase in allergen-specific IgE and ex vivo Th2 responses after a single bronchial challenge with house dust mite in allergic asthmatics

• Published in: Allergy (Copenhagen) 2012-01, Vol.67 (1), p.67-73
• Main Authors: van de Pol, M. A., Lutter, R., van Ree, R., van der Zee, J. S.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; allergen challenge; Allergens; allergic asthma; Allergies; Animals; Antigens, Dermatophagoides - immunology; Arthropod Proteins - immunology; Asthma; Asthma - immunology; Asthma - microbiology; Biological and medical sciences; Bronchial Provocation Tests; Chronic obstructive pulmonary disease, asthma; Cysteine Endopeptidases - immunology; Cytokines; Cytokines - blood; Cytokines - immunology; Dander; Dermatology; Dermatophagoides pteronyssinus; Double-Blind Method; Dust; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Grasses; Helper cells; Humans; Hypersensitivity; Hypersensitivity - immunology; Hypersensitivity - microbiology; Immunoglobulin E; Immunoglobulin E - blood; Immunoglobulin E - immunology; Immunoglobulins; Inflammation; Interleukin 13; Interleukin 4; Interleukin 5; Lung; lymphocytes; Lymphocytes T; Male; Medical sciences; Middle Aged; Peripheral blood mononuclear cells; Pneumology; Pollen; Pyroglyphidae - immunology; Respiratory tract; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Serum levels; Th2 Cells - immunology; Th2 response; Young Adult
• ISSN: 0105-4538; 1398-9995
• DOI: 10.1111/j.1398-9995.2011.02722.x

To cite this article: van de Pol MA, Lutter R, van Ree R, van der Zee JS. Increase in allergen‐specific IgE and ex vivo Th2 responses after a single bronchial challenge with house dust mite in allergic asthmatics.Allergy 2012; 67: 67–73. Background:  Airway responsiveness to allergen in patients with allergic asthma is studied by bronchial allergen challenge. Although the typical features of the early and late responses on lung function and bronchial inflammation after allergen challenge are well known, little has been reported as yet on any changes in systemic allergic and immunologic parameters after 4–6 weeks. Methods:  In a clinical study, 27 subjects with allergic asthma and house dust mite (HDM) allergy underwent a bronchial allergen challenge with HDM. Blood samples were collected before and 5 weeks after allergen challenge. Serum levels of total IgE and allergen‐specific IgE were measured, and peripheral blood mononuclear cells were isolated and stimulated ex vivo with HDM to determine the allergen‐specific T‐cell cytokine response. Results:  Five weeks after bronchial allergen challenge with HDM, the amount of circulating IgE against HDM and the major allergenic components Der p1 and Der p2 was significantly increased (10.8% and 8.8%, respectively). IgE antibodies against other environmental allergens decreased (grass pollen) or remained unchanged (cat dander). Allergen‐induced Th2‐cytokine production was also significantly increased (P