The molecular mechanism of action of bactericidal gold nanoparticles on Escherichia coli

Abstract This work examines the molecular mechanism of action of a class of bactericidal gold nanoparticles (NPs) which show potent antibacterial activities against multidrug-resistant Gram-negative bacteria by transcriptomic and proteomic approaches. Gold NPs exert their antibacterial activities ma...

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Bibliographic Details
Published in:Biomaterials 2012-03, Vol.33 (7), p.2327-2333
Main Authors: Cui, Yan, Zhao, Yuyun, Tian, Yue, Zhang, Wei, Lü, Xiaoying, Jiang, Xingyu
Format: Article
Language:English
Subjects:
Advanced Basic Science
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Dentistry
Drug Resistance, Multiple - drug effects
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Gold - chemistry
Gold - pharmacology
Gold nanoparticle
Hydrogen Peroxide - chemistry
Materials Testing
Metal Nanoparticles - chemistry
Microarray Analysis
Microbial Sensitivity Tests
Molecular mechanism
Oxidants - chemistry
Reactive Oxygen Species - metabolism
ROS
Transcriptomic/proteomic analysis
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Summary:Abstract This work examines the molecular mechanism of action of a class of bactericidal gold nanoparticles (NPs) which show potent antibacterial activities against multidrug-resistant Gram-negative bacteria by transcriptomic and proteomic approaches. Gold NPs exert their antibacterial activities mainly by two ways: one is to collapse membrane potential, inhibiting ATPase activities to decrease the ATP level; the other is to inhibit the subunit of ribosome from binding tRNA. Gold NPs enhance chemotaxis in the early-phase reaction. The action of gold NPs did not include reactive oxygen species (ROS)-related mechanism, the cause for cellular death induced by most bactericidal antibiotics and nanomaterials. Our investigation would allow the development of antibacterial agents that target the energy-metabolism and transcription of bacteria without triggering the ROS reaction, which may be at the same time harmful for the host when killing bacteria.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.11.057