Protective effect of chitosan oligosaccharides against Fc epsilon RI-mediated RBL-2H3 mast cell activation

The activation of mast cells by immunoglobulin E-mediated stimuli is considered as a central event in allergic responses. In this regard, chitosan oligosaccharides (COS) of two different molecular weight ranges (1-3 kDa and 3-5 kDa) were investigated for their capabilities against the activation of...

Full description

Saved in:
Bibliographic Details
Published in:Process biochemistry (1991) 2012-02, Vol.47 (2), p.327-330
Main Authors: Vo, Thanh-Sang, Kim, Jung-Ae, Ngo, Dai-Hung, Kong, Chang-Suk, Kim, Se-Kwon
Format: Article
Language:English
Subjects:
Activation
Antigens
Chitosan
Immunoglobulins
Inhibitors
Masts
Oligosaccharides
Stimuli
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The activation of mast cells by immunoglobulin E-mediated stimuli is considered as a central event in allergic responses. In this regard, chitosan oligosaccharides (COS) of two different molecular weight ranges (1-3 kDa and 3-5 kDa) were investigated for their capabilities against the activation of RBL-2H3 mast cell sensitized with dinitrophenyl-specific immunoglobulin E antibody and stimulated by antigen dinitrophenyl-bovine serum albumin. It was found that COS significantly inhibited RBL-2H3 cell degranulation via attenuating the releases of histamine and beta -hexosaminidase. Moreover, the inhibitory activity of COS was accompanied by a reduction in intracellular Ca2+ elevation. Notably, the expression of immunoglobulin Fc epsilon receptor I (Fc epsilon RI) in RBL-2H3 cells was down-regulated by COS treatment in a dose-dependent manner. The suppressive effect of COS on RBL-2H3 cell activation suggested that COS may be potential candidates of novel inhibitors against allergic reactions.
ISSN:1359-5113
DOI:10.1016/j.procbio.2011.10.036