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Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli
► Screening of antagonistic bacteria for new detergent proteases. ► Encapsulation of cells in alginate beads for induction of protease activity. ► Cloning of four extracellular proteases from Stenotrophomonas maltophilia. ► Modifying of proteases for soluble expression in E. coli. A large strain col...
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Published in: | Journal of biotechnology 2012-01, Vol.157 (1), p.140-147 |
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Main Authors: | , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | ► Screening of antagonistic bacteria for new detergent proteases. ► Encapsulation of cells in alginate beads for induction of protease activity. ► Cloning of four extracellular proteases from Stenotrophomonas maltophilia. ► Modifying of proteases for soluble expression in E. coli.
A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3.
From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45°C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17±2U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2. |
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ISSN: | 0168-1656 1873-4863 |
DOI: | 10.1016/j.jbiotec.2011.09.025 |