Dissemination of the rmtB gene carried on IncF and IncN plasmids among Enterobacteriaceae in a pig farm and its environment

• Published in: Journal of antimicrobial chemotherapy 2011-11, Vol.66 (11), p.2475-2479
• Main Authors: Yao, Qiongfen, Zeng, Zhenling, Hou, Jianxia, Deng, Yuting, He, Liangying, Tian, Wei, Zheng, Hongqing, Chen, Zhangliu, Liu, Jian-Hua
• Format: Article
• Language: English
• Subjects: Aminoglycosides - pharmacology; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antimicrobial agents; Bacteria; Bacterial Proteins - genetics; Biological and medical sciences; Disease transmission; Drug Resistance, Bacterial - genetics; Enterobacter aerogenes; Enterobacter cloacae; Enterobacteriaceae; Enterobacteriaceae - drug effects; Enterobacteriaceae - genetics; Enterobacteriaceae - isolation & purification; Escherichia coli; Escherichia coli Proteins - genetics; Gene Transfer, Horizontal; Hogs; Leclercia adecarboxylata; Medical sciences; Methyltransferases - genetics; Microbial Sensitivity Tests; Morganella morganii; Pharmacology. Drug treatments; Plasmids; Plasmids - genetics; Polymorphism, Restriction Fragment Length; Proteus mirabilis; RNA, Ribosomal, 16S - genetics; Soil Microbiology; Sus scrofa; Swine houses
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dkr328

Objectives To investigate the prevalence and characterization of 16S rRNA methylase-producing bacteria in a pig farm and its environment in East China. Methods Enterobacteriaceae isolates and metagenomic DNA from 102 pig faecal samples from a pig farm and 97 soil samples taken in or around the farm were screened for the presence of 16S rRNA methylase genes. The clonal relationships of 16S rRNA methylase-positive isolates, plasmid content and other associated resistance genes were also characterized. Results Fifty-six rmtB-positive Enterobacteriaceae isolates, including 54 Escherichia coli, 1 Morganella morganii and 1 Proteus mirabilis, were recovered from 55 pig faecal samples. Nineteen rmtB-positive bacteria, including 13 E. coli, 2 M. morganii, 2 Leclercia adecarboxylata, 1 Enterobacter aerogenes and 1 Enterobacter cloacae, were recovered from 16 soil samples. Among the 75 rmtB-positive isolates, 31 and 25 also carried the qepA and bla CTX-M genes, respectively. The qepA gene co-localized with rmtB on the F2:A-:B1 plasmids and the bla CTX-M-65 gene co-localized with rmtB on the F33:A-:B- plasmids. The rmtB gene was also found to be associated with the IncN plasmids. Clonal transmission of rmtB-positive E. coli isolates was observed between different pig groups and soil samples. Conclusions Both horizontal gene transfer and clonal spread could be responsible for the dissemination of the rmtB gene in the pig farm and its environment. To our knowledge, this study is the first report of rmtB-positive bacteria from farmland soils and indicates that these antibiotic-resistant bacteria and/or resistance genes could be acquired by humans through the food chain.