Efficient production of l-lactic acid from xylose by a recombinant Candida utilis strain

Efficient l-lactic acid production from xylose was achieved using a pyruvate decarboxylase-deficient Candida utilis strain expressing an l-lactate dehydrogenase, an NADH-preferring mutated xylose reductase (XR), a xylitol dehydrogenase and a xylulokinase. The recombinant strain showed 53% increased...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2012, Vol.113 (1), p.73-75
Main Authors: Tamakawa, Hideyuki, Ikushima, Shigehito, Yoshida, Satoshi
Format: Article
Language:English
Subjects:
Aldehyde Reductase - genetics
Aldehyde Reductase - metabolism
Biological and medical sciences
Biotechnology
Candida - enzymology
Candida - genetics
Candida - metabolism
Candida utilis
Culture Media - chemistry
D-Xylulose Reductase - genetics
D-Xylulose Reductase - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Industrial Microbiology
L-Lactate dehydrogenase
L-Lactate Dehydrogenase - genetics
L-Lactate Dehydrogenase - metabolism
l-lactic acid
Lactic Acid - biosynthesis
Mutagenesis, Site-Directed
Organisms, Genetically Modified - metabolism
Phosphotransferases (Alcohol Group Acceptor) - genetics
Phosphotransferases (Alcohol Group Acceptor) - metabolism
Plasmids
pyruvic acid
Temperature
xylitol
Xylitol dehydrogenase
Xylose
Xylose - metabolism
Xylose reductase
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Summary:Efficient l-lactic acid production from xylose was achieved using a pyruvate decarboxylase-deficient Candida utilis strain expressing an l-lactate dehydrogenase, an NADH-preferring mutated xylose reductase (XR), a xylitol dehydrogenase and a xylulokinase. The recombinant strain showed 53% increased l-lactic acid production compared with the reference strain expressing native XR (NADPH-preferring).
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2011.09.002