Evaluation of VIDAS® Salmonella (SLM) Easy Salmonella Method for the Detection of Salmonella in a Variety of Foods: Collaborative Study

• Published in: Journal of AOAC International 2011-11, Vol.94 (6), p.1821-1834
• Main Authors: CROWLEY, Erin, BIRD, Patrick, FISHER, Kiel, GOETZ, Katherine, BENZINGER, M. Joseph, AGIN, James, GOINS, David, JOHNSON, Ronald L
• Format: Article
• Language: English
• Subjects: Animals; Arachis - microbiology; Biological and medical sciences; Chemical properties; Cooperative Behavior; Crustacea - microbiology; Eggs - microbiology; Enzyme-Linked Immunosorbent Assay - instrumentation; Enzyme-Linked Immunosorbent Assay - methods; Fluorescence; Food contamination; Food industries; Food microbiology; Food Microbiology - instrumentation; Food Microbiology - methods; Fundamental and applied biological sciences. Psychology; General aspects; Humans; Ice Cream - microbiology; Identification and classification; Immunoassay; Laboratories; Methods; Methods of analysis, processing and quality control, regulation, standards; Poultry Products - microbiology; Reproducibility of Results; Salmonella; Salmonella - isolation & purification; Sensitivity and Specificity; Spinacia oleracea - microbiology; United States; United States Food and Drug Administration
• ISSN: 1060-3271; 1944-7922
• DOI: 10.5740/jaoacint.CS2011_03

The VIDAS Salmonella (SLM) Easy Salmonella method is a specific enzyme-linked fluorescent immunoassay performed in the automated VIDAS instrument. The VIDAS Easy Salmonella method is a simple 2-step enrichment procedure, using pre-enrichment followed by selective enrichment in a newly formulated broth, SX2 broth. This new method was compared in a multilaboratory collaborative study to the U.S. Food and Drug Administration's Bacteriological Analytical Manual, Chapter 5 method for five food matrixes (liquid egg, vanilla ice cream, spinach, raw shrimp, and peanut butter) and the U.S. Department of Agriculture's Microbiology Laboratory Guidebook 4.04 method for deli turkey. Each food type was artificially contaminated with Salmonella at three inoculation levels. A total of 15 laboratories representing government, academia, and industry, throughout the United States, participated. In this study, 1583 samples were analyzed, of which 792 were paired replicates and 791 were unpaired replicates. Of the 792 paired replicates, 285 were positive by both the VIDAS and reference methods. Of the 791 unpaired replicates, 341 were positive by the VIDAS method and 325 were positive by the cultural reference method. A Chi-square analysis of each of the six food types was performed at the three inoculation levels tested. For all foods evaluated, the VIDAS Easy SLM method demonstrated results comparable to those of the reference methods for the detection of Salmonella.