Flagellin Enhances Saliva IgA Response and Protection of Anti-caries DNA Vaccine

We and others have shown that anti-caries DNA vaccines, including pGJA-P/VAX, are promising for preventing dental caries. However, challenges remain because of the low immunogenicity of DNA vaccines. In this study, we used recombinant flagellin protein derived from Salmonella (FliC) as a mucosal adj...

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Bibliographic Details
Published in:Journal of dental research 2012-03, Vol.91 (3), p.249-254
Main Authors: Shi, W., Li, Y.H., Liu, F., Yang, J.Y., Zhou, D.H., Chen, Y.Q., Zhang, Y., Yang, Y., He, B.X., Han, C., Fan, M.W., Yan, H.M.
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Administration, Intranasal
Animals
Antibodies, Bacterial - immunology
Antigens
Colonization
Dental caries
Dental Caries - prevention & control
Dentistry
Deoxyribonucleic acid
DNA
DNA vaccines
Female
Flagellin
Flagellin - immunology
FliC protein
Immunization
Immunogenicity
Immunoglobulin A
Immunoglobulin A, Secretory - immunology
Immunoglobulin G
Infections
Laboratory animals
Mucosa
Physiology
Plasmids
Proteins
Rats
Rats, Wistar
Recombinant Proteins - immunology
Rodents
Saliva
Salivary Proteins and Peptides - immunology
Salmonella
Salmonella - immunology
Streptococcus infections
Streptococcus mutans - immunology
Sucrose
Teeth
Vaccines
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Virology
Virulence Factors
West Nile virus
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Summary:We and others have shown that anti-caries DNA vaccines, including pGJA-P/VAX, are promising for preventing dental caries. However, challenges remain because of the low immunogenicity of DNA vaccines. In this study, we used recombinant flagellin protein derived from Salmonella (FliC) as a mucosal adjuvant for anti-caries DNA vaccine (pGJA-P/VAX) and analyzed the effects of FliC protein on the serum PAc-specific IgG and saliva PAc-specific IgA antibody responses, the colonization of Streptococcus mutans (S. mutans) on rat teeth, and the formation of caries lesions. Our results showed that FliC promoted the production of PAc-specific IgG in serum and secretory IgA (S-IgA) in saliva of rats by intranasal immunization with pGJA-P/VAX plus FliC. Furthermore, we found that enhanced PAc-specific IgA responses in saliva were associated with the inhibition of S. mutans colonization of tooth surfaces and endowed better protection with significant fewer caries lesions. In conclusion, our study demonstrates that recombinant FliC could enhance specific IgA responses in saliva and protective ability of pGJA-P/VAX, providing an effective mucosal adjuvant candidate for intranasal immunization of an anti-caries DNA vaccine.
ISSN:0022-0345
1544-0591
DOI:10.1177/0022034511424283