Diversity and distribution of the bioactive actinobacterial genus Salinispora from sponges along the Great Barrier Reef

Isolates from the marine actinobacterial genus Salinispora were cultured from marine sponges collected from along the length of the Great Barrier Reef (GBR), Queensland, Australia. Strains of two species of Salinispora , Salinispora arenicola and “ Salinispora pacifica” , were isolated from GBR spon...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2012-03, Vol.101 (3), p.603-618
Main Authors: Vidgen, M. E., Hooper, J. N. A., Fuerst, J. A.
Format: Article
Language:English
Subjects:
Animals
Australia
Bacteria
Bacterial Proteins - genetics
Base Sequence
Biodiversity
Biological and medical sciences
Biomedical and Life Sciences
Coral Reefs
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Life Sciences
Medical Microbiology
Microbiology
Micromonosporaceae - classification
Micromonosporaceae - genetics
Micromonosporaceae - isolation & purification
Molecular Sequence Data
Nucleic Acid Conformation
Original Paper
Phylogeny
Plant Sciences
Porifera - microbiology
Ribonuclease P - genetics
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Sequence Alignment
Sequence Homology, Nucleic Acid
Soil Science & Conservation
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Summary:Isolates from the marine actinobacterial genus Salinispora were cultured from marine sponges collected from along the length of the Great Barrier Reef (GBR), Queensland, Australia. Strains of two species of Salinispora , Salinispora arenicola and “ Salinispora pacifica” , were isolated from GBR sponges Dercitus xanthus , Cinachyrella australiensis and Hyattella intestinalis . Phylogenetic analysis of the 16S rRNA gene sequences of representative strains, selected via BOX-PCR screening, identified previously unreported phylotypes of the species “ S.   pacifica ”. The classification of these microdiverse 16S rRNA groups was further confirmed by analysis of the ribonuclease P RNA (RNase P RNA) gene through both phylogenetic and secondary structure analysis. The use of RNase P RNA sequences combined with 16S rRNA sequences allowed distinction of six new intraspecies phylotypes of “ S. pacifica ” within the geographical area of the GBR alone. One of these new phylotypes possessed a localised regional distribution within the GBR.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-011-9676-9