Effects of resinous monomers on the odontogenic differentiation and mineralization potential of highly proliferative and clonogenic cultured apical papilla stem cells

• Published in: Dental materials 2012-03, Vol.28 (3), p.327-339
• Main Authors: Bakopoulou, Athina, Leyhausen, Gabriele, Volk, Joachim, Koidis, Petros, Geurtsen, Werner
• Format: Article
• Language: English
• Subjects: Adolescent; Adult Stem Cells - drug effects; Advanced Basic Science; Alkaline Phosphatase - biosynthesis; Apical papilla stem cells; Biomineralization; Cell Differentiation - drug effects; Cells, Cultured; Colony-Forming Units Assay; Dental pulp repair; Dentistry; Extracellular Matrix Proteins - biosynthesis; Flow Cytometry; Humans; Integrin-Binding Sialoprotein - biosynthesis; Methacrylates - toxicity; Odontogenesis - drug effects; Odontogenic differentiation; Osteocalcin - biosynthesis; Phosphoproteins - biosynthesis; Polyethylene Glycols - toxicity; Polymethacrylic Acids - toxicity; Resinous monomers; Sialoglycoproteins - biosynthesis; Tooth Apex - cytology; Tooth Apex - drug effects; Tooth Calcification - drug effects
• ISSN: 0109-5641; 1879-0097
• DOI: 10.1016/j.dental.2012.01.002

Abstract Objective The aim of this study was to investigate the effects of resinous monomers on the odontogenic differentiation and mineralization potential of apical papilla stem cells (SCAP). Methods Cultures were established from developing third molars of healthy donors aged 14–18 years-old and were extensively characterized for proliferation rate, colony forming unit efficiency and expression of stem cell markers (STRO-1, CD146, CD34, CD45, CD105, CD117-c-Kit, CD24, CD90, Nanog, Oct3/4), in order to select those with enhanced stem cell and odontogenic differentiation properties. SCAP enriched cultures were then induced for odontogenic differentiation in the continuous presence of low concentrations (0.05–0.5 mM) of the monomers 2-hydroxy-ethyl-methacrylate-HEMA and triethylene-glycol-dimethacrylate-TEGDMA for 3 weeks (long-term exposure). Additionally, the effects of a single exposure (72 h) to higher concentrations of HEMA (2 mM) and TEGDMA (1 mM) were evaluated. Results The results showed that both types of monomer-exposure significantly delayed the odontogenic differentiation and mineralization processes of SCAP cells. A down-regulation followed by recovery in the expression of differentiation markers, including dentin sialophosphoprotein-DSPP, bone sialoprotein-BSP, osteocalcin-OCN and alkaline phosphatase-ALP was recorded. This was accompanied by reduction of the mineralized matrix produced by monomer-treated-compared to non-treated contol cultures. Furthermore, a concentration-dependence was observed for both monomers during long-term exposure, whereas the effects of HEMA were evident at much lower concentrations compared to TEGDMA. Significance These findings suggest that resinous monomers can delay the odontogenic differentiation of SCAP cells, potentially disturbing the physiological repair and/or developmental processes of human permanent teeth.