Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA

• Published in: FEBS letters 2012-02, Vol.586 (4), p.356-361
• Main Authors: Seong, Ki Moon, Jung, Sang-Oun, Kim, Hag Dong, Kim, Hee Ju, Jung, You-Jin, Choi, Soo-Young, Kim, Joon
• Format: Article
• Language: English
• Subjects: 8-Oxo-G; AP sites; DNA Damage; DNA repair; DNA Repair - genetics; DNA Repair - physiology; DNA, Fungal - metabolism; DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics; DNA-(Apurinic or Apyrimidinic Site) Lyase - metabolism; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Genetic Complementation Test; Kinetics; Lyase; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Pyrimidine dimer; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sequence Deletion; Substrate Specificity; Yeast Rps3p
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2011.12.030

► Yeast Rps3p cleaves not only AP sites and pyrimidine dimers but also 8-oxo-G. ► Yeast Rps3p could cleave pyrimidine dimers without glycosylase activity. ► Asp154 and Lys200 are identified as important residues for the catalytic activity. Yeast ribosomal protein S3 has multifunctional activities that are involved in both protein translation and DNA repair. Here, we report that yeast Rps3p cleaves variously damaged DNA that contains not only AP sites and pyrimidine dimers but also 7,8-hydro-8-oxoguanine. This study also revealed that Rps3p has a β-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates. Mutation analysis of the yeast Rps3 protein including introduction of domain deletions and residue replacements identified the residues Asp154 and Lys200 are important for the catalytic activity. In addition, the repair enzyme activity of yeast Rps3p was confirmed by complementation in xth, nfo Escherichia coli cells in which the DNA repair process is defective.