Human herpesvirus 6A infects human embryonic fibroblasts and induces G2/M arrest and cell death

Human herpesvirus 6 (HHV‐6) is a beta‐herpesvirus capable of infecting cells from different origin. In this study, infection with HHV‐6A of human embryonic fibroblasts (HEFs) was performed. Infected cells showed obvious cytopathic effects (CPE). PCR and immunohistochemical tests also confirmed that...

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Bibliographic Details
Published in:Journal of medical virology 2012-04, Vol.84 (4), p.657-663
Main Authors: Li, Lingyun, Gu, Bin, Zhou, Feng, Chi, Jing, Wang, Fang, Liu, Genyan, Ding, Chuanlin, Xie, Fangyi, Qing, Jian, Guo, Yidi, Yao, Kun
Format: Article
Language:English
Subjects:
Biological and medical sciences
cell cycle arrest
Cell Death
Cell Division
Cell Proliferation
Cells, Cultured
Cytopathogenic Effect, Viral
Fibroblasts - pathology
Fibroblasts - virology
Fundamental and applied biological sciences. Psychology
Herpesvirus 6, Human - pathogenicity
human embryonic fibroblasts
Human herpesvirus 6
Human viral diseases
Humans
Immunohistochemistry
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Polymerase Chain Reaction
Viral diseases
Virology
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Summary:Human herpesvirus 6 (HHV‐6) is a beta‐herpesvirus capable of infecting cells from different origin. In this study, infection with HHV‐6A of human embryonic fibroblasts (HEFs) was performed. Infected cells showed obvious cytopathic effects (CPE). PCR and immunohistochemical tests also confirmed that HEFs are susceptible to HHV‐6A infection. The biological effects of HHV‐6A infection on HEFs were studied. Infected cells showed decreased proliferation as measured by [3H] thymidine incorporation and cell counting. Further analysis demonstrated that infection with HHV‐6A leads to cell cycle arrest at G2/M phase and increasing cell death. This is the first demonstration that infection of HEFs with HHV‐6A causes profound alterations of cell properties. J. Med. Virol. 84:657–663, 2012. © 2011 Wiley Periodicals, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.23226