Fish meal supplementation increases bovine plasma and luteal tissue omega-3 fatty acid composition

• Published in: Journal of animal science 2012-03, Vol.90 (3), p.771-778
• Main Authors: White, N. R, Burns, P. D, Cheatham, R. D, Romero, R. M, Nozykowski, J. P, Bruemmer, J. E, Engle, T. E
• Format: Article
• Language: English
• Subjects: Angus; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animals; arachidonic acid; blood; Body Weight; Cattle - blood; corn; corn gluten meal; corpus luteum; Corpus Luteum - chemistry; cows; diet; Diet - veterinary; Dinoprost; docosahexaenoic acid; eicosapentaenoic acid; Estrus Synchronization; fatty acid composition; Fatty Acids, Omega-3 - blood; Fatty Acids, Omega-3 - metabolism; Female; fish; fish meal; Fish Products; omega-3 fatty acids
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/jas.2011-4208

The objective of this experiment was to determine if dietary inclusion of fish meal would increase plasma and luteal tissue concentrations of eicosapentaenoic and docosahexaenoic acids. Seventeen nonlactating Angus cows (2 to 8 yr of age) were housed in individual pens and fed a corn silage-based diet for approximately 60 d. Diets were supplemented with fish meal at 5% DMI (a rich source of eicosapentaenoic acid and docosahexaenoic acid; n = 9 cows) or corn gluten meal at 6% DMI (n = 8 cows). Body weights and jugular blood samples were collected immediately before the initiation of supplementation and every 7 d thereafter for 56 d to monitor plasma n-3 fatty acid composition and BW. Estrous cycles were synchronized using 2 injections of PGF2α administered at 14-d intervals. The ovary bearing the corpus luteum was surgically removed at midcycle (between d 10 and 12) after estrus synchronization, which corresponded to approximately d 60 of supplementation. The ovary was transported to the laboratory, and approximately 1.5 g of luteal tissue was stored at –80°C until analyzed for n-3 fatty acid content. Initial and ending BW did not differ (P > 0.10) between cows supplemented with fish meal and those with corn gluten meal. Plasma eicosapentaenoic acid was greater (P < 0.05) beginning at d 7 of supplementation and docosahexaenoic was greater (P < 0.05) beginning at d 14 of supplementation for cows receiving fish meal. Luteal tissue collected from fish meal-supplemented cows had greater (P < 0.05) luteal n-3 fatty acids and reduced (P < 0.05) arachidonic acid and n-6 to n-3 ratio as compared with tissue obtained from cows supplemented with corn gluten meal. Our data show that fish meal supplementation increases luteal n-3 fatty acid content and reduces available arachidonic acid content, the precursor for PGF2α. The increase in luteal n-3 fatty acids may reduce PGF2α intraluteal synthesis after breeding resulting in increased fertility in cattle.