Transplantation of Ex Vivo Expanded Bone Marrow-Derived Endothelial Progenitor Cells Enhances Chronic Venous Thrombus Resolution and Recanalization

Background: Preclinical and clinical studies indicated that endothelial progenitor cells (EPCs) enhanced blood vessel formation in many clinical situations. However, whether transplantation of EPCs would enhance chronic venous thrombus recanalization and resolution is unknown. Methods: Mononuclear c...

Full description

Saved in:
Bibliographic Details
Published in:Clinical and applied thrombosis/hemostasis 2011-11, Vol.17 (6), p.E196-E201
Main Authors: Meng, Qingyou, Li, Xiaoqiang, Yu, Xiaobin, Lei, Fengrui, Jiang, Kun, Li, Chuanyong
Format: Article
Language:English
Subjects:
Animals
Blood clots
Bone marrow
Bone Marrow Transplantation - methods
Catheters, Indwelling
Disease Models, Animal
Endothelial Cells - cytology
Endothelial Cells - transplantation
Humans
Immunohistochemistry
Male
Protein expression
Rats
Rats, Wistar
Stem Cell Transplantation - methods
Stem Cells - cytology
Thrombosis - metabolism
Thrombosis - pathology
Thrombosis - surgery
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - blood
Vascular Endothelial Growth Factor A - metabolism
Venous Thrombosis - metabolism
Venous Thrombosis - surgery
Venous Thrombosis - therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Preclinical and clinical studies indicated that endothelial progenitor cells (EPCs) enhanced blood vessel formation in many clinical situations. However, whether transplantation of EPCs would enhance chronic venous thrombus recanalization and resolution is unknown. Methods: Mononuclear cells were isolated from bone marrow of immature rats by density gradient centrifugation, cultured, and then transplanted into inferior vena cava of rats with experimentally induced thrombi. Vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), messenger RNA (mRNA), and protein expression levels were measured through real-time quantitative polymerase chain reaction and Western blotting of thrombi and adjacent caval walls 14 days following transplantation. Results: Transplantation of bone marrow-derived EPCs led to an increase in VEGF, bFGF, mRNA, and protein expression. In addition, transplantation of bone marrow-derived EPCs also resulted in reduced thrombus size and increased neovascularization in the specimen. Conclusions: Transplanted bone marrow-derived EPCs may be a therapeutic option for treating deep venous thrombosis.
ISSN:1076-0296
1938-2723
DOI:10.1177/1076029610397180